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首页> 外文期刊>Applied Microbiology and Biotechnology >Simultaneous detection and removal of organomercurial compounds by using the genetic expression system of an organomercury lyase from the transposon TnMERI1
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Simultaneous detection and removal of organomercurial compounds by using the genetic expression system of an organomercury lyase from the transposon TnMERI1

机译：通过转座子TnMERI1的有机汞裂解酶基因表达系统，同时检测和去除有机汞化合物

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Using a newly identified organomercury lyase gene (merB3) expression system from TnMERII. the mercury resistance transposon first found in Gram-positive bacteria, a dual-purpose system to detect and remove organomercurial contamination was developed. A plasmid was constructed by fusing the promoterless lux-AB genes as bioluminescence reporter genes downstream of the merB3 gene and its operator/promoter region. Another plasmid, encoding mer operon genes from merR1 to merA, was also constructed to generate an expression regulatory protein, MerR1, and a mercury reductase enzyme, MerA. These two plasmids were transformed into Escherichia coli cells to produce a biological system that can detect and remove environmental organomercury contamination. Organomercurial compounds, such as neurotoxic methylmercury at nanomolar levels, were detected using the biomonitoring system within a few minutes and were removed during the next few hours. [References: 21]
机译：使用来自TnMERII的新鉴定的有机汞裂解酶基因（merB3）表达系统。最早在革兰氏阳性细菌中发现的抗汞转座子，开发了一种用于检测和去除有机汞污染的双重用途系统。通过融合无启动子的lux-AB基因作为merB3基因及其操纵子/启动子区域下游的生物发光报告基因来构建质粒。还构建了另一种编码从merR1到merA的mer操纵子基因的质粒，以生成表达调节蛋白MerR1和汞还原酶MerA。将这两个质粒转化到大肠杆菌细胞中，以产生可以检测和去除环境有机汞污染的生物系统。使用生物监测系统在几分钟内检测到了有机汞化合物，例如纳摩尔水平的神经毒性甲基汞，并在接下来的几个小时内将其去除。 [参考：21]

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