Flocculent activity of a recombinant protein from Moringa oleifera Lam. seeds

M. Broin; C. Santaella; S. Cuine; K. Kokou; G. Peltier; T. Joët

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Flocculent activity of a recombinant protein from Moringa oleifera Lam. seeds

机译：辣木重组蛋白的絮凝活性。种子

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Seeds of the tropical tree Moringa oleifera contain small storage proteins able to flocculate particles in suspension in water. The cDNA encoding one of these flocculent proteins, MO2.1, was cloned and the recombinant protein was expressed in Escherichia coli. The flocculent activity of the purified recombinant MO2.1 was assayed on clays and bacteria using light and confocal microscopy and GFP-overexpressing bacteria. We show that MO2.1 is able to aggregate montmorillonite clay particles as well as gram-positive and gram-negative bacteria. We discuss the use of recombinant proteins to study flocculating properties and improve water purification processes.

机译：热带树木辣木的种子含有小的储存蛋白，能够使悬浮在水中的颗粒絮凝。克隆了一种编码这些絮凝蛋白的cDNA MO2.1 ，并在大肠杆菌中表达了该重组蛋白。使用光学和共聚焦显微镜以及过表达GFP的细菌，测定了纯化的重组MO2.1 在黏土和细菌上的絮凝活性。我们证明MO2.1 能够聚集蒙脱土粘土颗粒以及革兰氏阳性和革兰氏阴性细菌。我们讨论了使用重组蛋白研究絮凝特性和改善水净化过程的方法。

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《Applied Microbiology and Biotechnology》 |2002年第2期|114-119|共6页
作者
M. Broin; C. Santaella; S. Cuine; K. Kokou; G. Peltier; T. Joët;
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CEA/Cadarache Direction des Sciences du Vivant Département d'Ecophysiologie Végétale et Microbiologie Laboratoire d'Ecophysiologie de la Photosynthèse UMR 163 CNRS CEA Univ-Méditerranée CEA 1000 13108 Saint-Paul-lez-Durance France;

CEA/Cadarache Direction des Sciences du Vivant Département d'Ecophysiologie Végétale et Microbiologie Laboratoire d'Ecologie Microbienne et de la Rhizosphère UMR 163 CNRS CEA Univ-Méditerranée 13108 Saint-Paul-lez-Durance France;

CEA/Cadarache Direction des Sciences du Vivant Département d'Ecophysiologie Végétale et Microbiologie Laboratoire d'Ecophysiologie de la Photosynthèse UMR 163 CNRS CEA Univ-Méditerranée CEA 1000 13108 Saint-Paul-lez-Durance France;

Laboratoire de Botanique et Ecologie Végétale Faculté des Sciences Université de Lomé BP1515 Lomé Togo;

CEA/Cadarache Direction des Sciences du Vivant Département d'Ecophysiologie Végétale et Microbiologie Laboratoire d'Ecophysiologie de la Photosynthèse UMR 163 CNRS CEA Univ-Méditerranée CEA 1000 13108 Saint-Paul-lez-Durance France;

CEA/Cadarache Direction des Sciences du Vivant Département d'Ecophysiologie Végétale et Microbiologie Laboratoire d'Ecophysiologie de la Photosynthèse UMR 163 CNRS CEA Univ-Méditerranée CEA 1000 13108 Saint-Paul-lez-Durance France;

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Flocculent activity of a recombinant protein from Moringa oleifera Lam. seeds

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