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首页> 外文期刊>Applied Microbiology and Biotechnology >Biodegradation of ethyl t-butyl ether (ETBE), methyl t-butyl ether (MTBE) and t-amyl methyl ether (TAME) by Gordonia terrae
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Biodegradation of ethyl t-butyl ether (ETBE), methyl t-butyl ether (MTBE) and t-amyl methyl ether (TAME) by Gordonia terrae

机译:Gordonia terrae对乙基叔丁基醚(ETBE),甲基叔丁基醚(MTBE)和叔戊基甲基醚(TAME)的生物降解

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摘要

Gordonia terrae strain IFP 2001 was selected from activated sludge for its capacity to grow on ethyl t-butyl ether (ETBE) as sole carbon and energy source. ETBE was stoichiometrically degraded to t-butyl alcohol (TBA) and the activity was inducible. A constitutive strain, G. terrae IFP 2007, derived from strain IFP 2001, was also selected. Methyl t-butyl ether (MTBE) and t-amyl methyl ether (TAME) were not used as carbon and energy sources by the two strains, but cometabolic degradation of MTBE and TAME was demonstrated, to TBA and t-amyl alcohol (TAA) respectively, in the presence of a carbon source such as ethanol. No two-carbon compound was detected during growth on ETBE, but formate was produced during cometabolic degradation of MTBE or TAME. A monooxygenase was involved in the degradation of ethers, because no degradation of ETBE was observed under anaerobic conditions and the presence of a cytochrome P-450 was demonstrated in G. terrae IFP 2001 after induction by cultivation on ETBE.
机译:从活性污泥中选择了Gordonia terra菌株IFP 2001,因为它具有在乙基叔丁基醚(ETBE)上作为唯一碳和能源的生长能力。 ETBE在化学计量上被降解为叔丁醇(TBA),并且其活性是可诱导的。还选择了源自菌株IFP 2001的本构菌株G.terrae IFP 2007。两种菌株均未将甲基叔丁基醚(MTBE)和叔戊基甲基醚(TAME)用作碳和能源,但已证明MTBE和TAME的可代谢降解为TBA和叔戊醇(TAA)分别在碳源(如乙醇)存在下。在ETBE上生长期间未检测到二碳化合物,但在MTBE或TAME的代谢降解过程中产生了甲酸盐。单加氧酶参与了醚的降解,因为在厌氧条件下未观察到ETBE的降解,并且通过在ETBE上诱导而在G.terrae IFP 2001中证实了细胞色素P-450的存在。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2001年第1期|117-121|共5页
  • 作者单位

    Institut Français du Pétrole Département de Microbiologie 1 et 4 avenue de Bois-Préau 92852 Rueil-Malmaison Cedex France;

    Institut Français du Pétrole Département de Microbiologie 1 et 4 avenue de Bois-Préau 92852 Rueil-Malmaison Cedex France;

    Institut Français du Pétrole Département de Microbiologie 1 et 4 avenue de Bois-Préau 92852 Rueil-Malmaison Cedex France;

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