Expression level of heterologous proteins in Pichia pastoris is influenced by flask design

F. Villatte; A. Hussein; T. Bachmann; R. Schmid

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Expression level of heterologous proteins in Pichia pastoris is influenced by flask design

机译：巴斯德毕赤酵母中异源蛋白的表达水平受烧瓶设计的影响

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The yeast Pichia pastoris is a convenient production system that enables expression of heterologous proteins in high amounts. As a fermentation method, shaking flasks are very popular because of their simplicity of handling and their low cost. We compared the expression level of the enzyme acetylcholinesterase in a transformed strain of P. pastoris grown in different flasks, presenting various designs but all with the same volume. A several-thousand-fold difference appeared in the expression levels; and the results could not be explained by differences between the flasks in the oxygenation of the medium. The data show that flask design is an important factor to consider for optimising fermentation processes.

机译：酵母巴斯德毕赤酵母是一种方便的生产系统，能够大量表达异源蛋白质。作为发酵方法，摇瓶由于其操作简单和成本低而非常受欢迎。我们比较了在不同烧瓶中生长的转化毕赤酵母菌株中乙酰胆碱酯酶的表达水平，提出了各种设计，但全部体积相同。表达水平出现了数千倍的差异。并且结果不能通过烧瓶之间介质氧合的差异来解释。数据表明烧瓶设计是优化发酵过程要考虑的重要因素。

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《Applied Microbiology and Biotechnology》 |2001年第4期|463-465|共3页
作者
F. Villatte; A. Hussein; T. Bachmann; R. Schmid;
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Institute für Technische Biochemie Allmandring 31 Stuttgart Universität 70569 Stuttgart Germany;

Department of Biochemistry Imperial College of Science Technology and Medicine London SW7 2AY United Kingdom;

Institute für Technische Biochemie Allmandring 31 Stuttgart Universität 70569 Stuttgart Germany;

Institute für Technische Biochemie Allmandring 31 Stuttgart Universität 70569 Stuttgart Germany;

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1. Expression level of heterologous proteins in Pichia pastoris is influenced by flask design [J] . Villatte F., Bachmann TT., Schmid RD., Applied Microbiology and Biotechnology . 2001,第4期

机译：巴斯德毕赤酵母中异源蛋白的表达水平受烧瓶设计的影响
2. A Mini-Scale Mass Production and Separation System for Secretory Heterologous Proteins by Perfusion Culture of Recombinant Pichia pastoris Using a Shaken Ceramic Membrane Flask [J] . Ryo Ohashi, Eiko Mochizuki, Takahiro Suzuki Journal of Bioscience and Bioengineering . 1999,第5期

机译：使用摇动的陶瓷膜瓶灌注重组毕赤酵母的微型大规模生产和分离分泌异源蛋白的系统。
3. Expression and One-Step Purification of Recombinant Proteins Using an Alternative Episomal Vector for the Expression of N-Tagged Heterologous Proteins in Pichia pastoris [J] . Cristiane Akemi Uchima, Manabu Arioka Bioscience, Biotechnology, and Biochemistry . 2012,第2期

机译：使用替代再生载体的重组蛋白的表达和一步纯化用于表达N标记的异源蛋白在Pichia Pastoris中的表达
4. Mass Spectrometric Analysis of the Secretion and Proteolysis of Heterologous Proteins in Pichia Pastoris [C] . Zhiguo Li, Wilson Leung, Joan Lin-Cereghino, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：毕赤酵母异源蛋白分泌和蛋白水解的质谱分析
5. Development of Secretion System for Heterologous Recombinant Protein Expression in E.coli Bacteria and Pichia pastoris Yeast. [D] . Qu, He. 2014

机译：大肠杆菌和巴斯德毕赤酵母中异源重组蛋白表达分泌系统的开发。
6. High-Level Heterologous Expression of Endo-14-β-Xylanase from Penicillium citrinum in Pichia pastoris X-33 Directed through Codon Optimization and Optimized Expression [O] . Chanika Ouephanit, Nassapat Boonvitthya, Sophie Bozonnet, 2019

机译：通过密码子优化和优化表达指导柠檬青霉中Pendolas X-33中柠檬酸内酯-14-β-木聚糖酶的高水平异源表达
7. Evaluation of antifoams in the expression of a recombinant FC fusion protein in shake flask cultures of Saccharomyces cerevisiae &Pichia pastoris [O] . Bill Roslyn, Smith Rodney, Holmes William 2006

机译：酿酒酵母＆巴斯德毕赤酵母的摇瓶培养物中消泡剂对重组FC融合蛋白表达的评价

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Expression level of heterologous proteins in Pichia pastoris is influenced by flask design

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