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首页> 外文期刊>Applied Microbiology and Biotechnology >Shear stress enhances microcin B17 production in a rotating wall bioreactor, but ethanol stress does not
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Shear stress enhances microcin B17 production in a rotating wall bioreactor, but ethanol stress does not

机译：剪应力可提高旋转壁式生物反应器中微素B17的产量，但乙醇胁迫不会

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Stress, including that caused by ethanol, has been shown to induce or promote secondary metabolism in a number of microbial systems. Rotating-wall bioreactors provide a low stress and simulated microgravity environment which, however, supports only poor production of microcin B17 by Escherichia coli ZK650, as compared to production in agitated flasks. We wondered whether the poor production is due to the low level of stress and whether increasing stress in the bioreactors would raise the amount of microcin B17 formed. We found that applying shear stress by addition of a single Teflon bead to a rotating wall bioreactor improved microcin B17 production. By contrast, addition of various concentrations of ethanol to such bioreactors (or to shaken flasks) failed to increase microcin B17 production. Ethanol stress merely decreased production and, at higher concentrations, inhibited growth. Interestingly, cells growing in the bioreactor were much more resistant to the growth-inhibitory and production-inhibitory effects of ethanol than cells growing in shaken flasks.
机译：压力（包括乙醇引起的压力）已显示出可以诱导或促进许多微生物系统的次级代谢。旋转壁生物反应器提供了低应力和模拟的微重力环境，但是，与搅拌烧瓶中的生产相比，大肠杆菌ZK650仅支持微素B17的不良生产。我们想知道产量不佳是否是由于低水平的压力造成的，以及生物反应器中压力的增加是否会增加所形成的微素B17的数量。我们发现通过向旋转壁生物反应器中添加单个特氟龙珠来施加剪切应力可以改善微素B17的生产。相比之下，向此类生物反应器（或摇瓶）中添加各种浓度的乙醇均无法增加microcin B17的产量。乙醇胁迫仅会降低产量，并且在较高浓度下会抑制生长。有趣的是，与在摇瓶中生长的细胞相比，在生物反应器中生长的细胞对乙醇的生长抑制和生产抑制作用更具抵抗力。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2001年第4期|384-387|共4页
  • 作者

    Q. Gao; A. Fang; D. Pierson; S. Mishra; A. Demain;

  • 作者单位

    Fermentation Microbiology Laboratory Department of Biology Massachusetts Institute of Technology Cambridge MA 02139 USA;

    Fermentation Microbiology Laboratory Department of Biology Massachusetts Institute of Technology Cambridge MA 02139 USA;

    NASA Johnson Space Center Houston TX 77058 USA;

    Universities Space Research Association Johnson Space Center Houston TX 77058 USA;

    Fermentation Microbiology Laboratory Department of Biology Massachusetts Institute of Technology Cambridge MA 02139 USA;

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