Synthesis of β-D-glucosyl- and β-D-fucosyl-glucoses using β-glycosidase from Thermus thermophilus

L. Fourage; B. Colas

首页> 外文期刊>Applied Microbiology and Biotechnology >Synthesis of β-D-glucosyl- and β-D-fucosyl-glucoses using β-glycosidase from Thermus thermophilus

【24h】

Synthesis of β-D-glucosyl- and β-D-fucosyl-glucoses using β-glycosidase from Thermus thermophilus

机译：用嗜热栖热菌的β-糖苷酶合成β-D-葡萄糖基和β-D-岩藻糖基葡萄糖

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The thermostable β-glycosidase from Thermus thermophilus, cloned and overexpressed in Escherichia coli was used to catalyze the transfer of β-D-glucosyl and β-D-fucosyl from the corresponding p-nitrophenyl-β-D-glycopyranosides to a hydroxyl group of glucose in the synthesis of β-D-glucosyl-D-glucopyranoses and β-D-fucosyl-D-glucopyranoses. The yields in disaccharides produced under conditions of non-initial velocity were very attractive and the formation of the β(1–3) linked disaccharides was largely favored. The enzyme could constitute a valuable biocatalyst for the synthesis of disaccharides involving such structures as, for example, Bifidus factors.

机译：在大肠杆菌中克隆并过表达的嗜热栖热菌的热稳定β-糖苷酶被用来催化β-D-葡萄糖基和β-D-岩藻糖基从相应的对硝基苯基-β-D-糖吡喃糖苷转移至羟基β-D-葡萄糖基-D-吡喃葡萄糖和β-D-岩藻糖基-D-吡喃葡萄糖的合成中使用葡萄糖。在非初始速度条件下产生的二糖收率非常诱人，β（1-3）连接的二糖的形成在很大程度上受到青睐。该酶可以构成有价值的生物催化剂，用于合成涉及诸如双歧因子等结构的二糖。

著录项

来源
《Applied Microbiology and Biotechnology》 |2001年第4期|406-410|共5页
作者
L. Fourage; B. Colas;
展开▼
作者单位

Laboratoire de Biochimie Unité de Recherche sur la Biocatalyse CNRS 2230 Faculté des Sciences et des Techniques Université de Nantes 2 rue de la Houssinière BP 92208 44322 Nantes Cedex 3 France;

Laboratoire de Biochimie Unité de Recherche sur la Biocatalyse CNRS 2230 Faculté des Sciences et des Techniques Université de Nantes 2 rue de la Houssinière BP 92208 44322 Nantes Cedex 3 France;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. Transferase Activity of a #beta#-Glycosidase from Thermus thermophilus: Specificities and Limits-Application to the Synthesis of #beta#-[1 -> 3]-Disaccharides [J] . Violaine Chiffoleau-Giranud, Petra Spangenberg, Michel Dion, European journal of organic chemistry . 1999,第4期

机译：来自嗜热栖热菌的＃beta＃-糖苷酶的转移酶活性：特异性和局限性-在合成#beta＃-[1-> 3]-二糖中的应用
2. Comparing Hydrolysis and Transglycosylation Reactions Catalyzed by Thermus thermophilus β-Glycosidase. A Combined MD and QM/MM Study [J] . Romero-Téllez Sonia, Lluch José M., González-Lafont àngels, Frontiers in Chemistry . 2019,第2004期

机译：嗜热栖热菌β-糖苷酶催化水解和糖基化反应的比较。 MD和QM / MM组合研究
3. Design of mutants for enhanced thermostability of β-glycosidase BglY from Thermus thermophilus [J] . Yi Z.-L., Zhang S.-B., Pei X.-Q., Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies . 2013,第Null期

机译：从热嗜热杆菌中增强β-糖苷酶的热稳定性的突变体的设计
4. Characterization and Functional Expression of Xylose Isomerase from Thermus thermophilus [C] . Angen Lu, ZexiYang, Fei Wang, International conference on biotechnology, chemical and materials engineering . 2013

机译：嗜热栖热菌木糖异构酶的表征和功能表达
5. Single Particle Electron Cryomicroscopy of the Thermus thermophilus V/A-Type Rotary ATPase and Structure of the Membrane-Bound a Subunit. [D] . Schep, Daniel. 2016

机译：嗜热栖热菌V / A型旋转ATPase的单粒子电子低温显微镜和膜结合a亚基的结构。
6. Comparing Hydrolysis and Transglycosylation Reactions Catalyzed by Thermus thermophilus β-Glycosidase. A Combined MD and QM/MM Study [O] . Sonia Romero-Téllez, José M. Lluch, Àngels González-Lafont, 1970

机译：嗜热栖热菌β-糖苷酶催化水解和糖基化反应的比较。 MD和QM / MM组合研究
7. Thermus thermophilus nucleoside phosphorylases active in the synthesis of nucleoside analogues [O] . Almendros, Marcos, Berenguer, José, Sinisterra, José Vicente 2015

机译：嗜热栖热菌核苷磷酸化酶在核苷类似物的合成中具有活性

Synthesis of β-D-glucosyl- and β-D-fucosyl-glucoses using β-glycosidase from Thermus thermophilus

摘要

著录项

相似文献

相关主题

期刊订阅