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首页> 外文期刊>Applied Microbiology and Biotechnology >Degradation of 2,4,6-trichlorophenol (2,4,6-TCP) by co-immobilization of anaerobic and aerobic microbial communities in an upflow reactor under air-limited conditions
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Degradation of 2,4,6-trichlorophenol (2,4,6-TCP) by co-immobilization of anaerobic and aerobic microbial communities in an upflow reactor under air-limited conditions

机译:在空气受限的条件下,通过将厌氧和好氧微生物群落共同固定在上流反应器中,降解2,4,6-三氯苯酚(2,4,6-TCP)

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摘要

The co-immobilization and the culture of anaerobic and aerobic communities was tested for the mineralization of 2,4,6-trichlorophenol (2,4,6-TCP). At first, the anaerobic microorganisms (aggregated into granules) were cultivated in an upflow anaerobic sludge blanket (UASB) reactor, in a continuous mode, with glucose, propionate, acetate (COD loading rate = 0.5–2.0 g COD/l per day, ratio 1:1:1) and 2,4,6-TCP (2,4,6-TCP loading rate = 25–278 µmol/l per day) as substrates. 2,4,6-TCP was degraded into 2,4-DCP and 4-CP, but it was not mineralized because of the low degradation rates of 4-CP. Furthermore, the highest loading rates of 2,4,6-TCP (>126 µmol/l per day) caused the inhibition of the strains degrading the propionate. The granules were therefore tested in association with the aerobic community. They were immobilized in κ-carrageenan/gelatin [2% (w/w) of each polymer] gel beads and cultivated in a reactor, on their own (to test the influence of the gel), and then with the aerobic community, under anaerobic and air-limited conditions, respectively. The results showed that (1) the gel did not influence the activity of the granules, (2) the anaerobic and aerobic communities could be easily co-immobilized in gel beads and cultivated in a reactor, (3) the mineralization of 2,4,6-TCP (2,4,6-TCP loading rate = 10–506 µmol/l per day), its intermediates of degradation and the other substrates [glucose + acetate + propionate (ratio 1:1:1) = COD loading rate = 500 mg COD/l per day] could be obtained under air-limited conditions if the culture parameters were strictly controlled [airflow = 36–48 vvd (volume of air/volume of liquid in the reactor per day), pH value at around 7.5]. Finally, the gel did not retain its structure during the whole culture (263 days) in the air-limited reactor, but the anaerobic and aerobic communities retained their activities and worked together for the mineralization.
机译:厌氧和好氧社区的共同固定化和文化进行了测试的2,4,6-三氯苯酚(2,4,6-TCP)的矿化。首先,将厌氧微生物(聚集成颗粒)在连续的厌氧污泥覆盖(UASB)反应器中以葡萄糖,丙酸酯和乙酸盐(每天的COD加载量= 0.5–2.0 g COD / l,比率1:1:1)和2,4,6-TCP(2,4,6-TCP加载速率= 25–278 µmol / l /天)作为底物。 2,4,6-TCP被降解为2,4-DCP和4-CP,但由于4-CP的降解率低而未被矿化。此外,最高的2,4,6-TCP加载速率(每天> 126 µmol / l)导致抑制了降解丙酸酯的菌株。因此,结合有氧社区对颗粒进行了测试。将它们固定在κ-角叉菜胶/明胶[每种聚合物的2%(w / w)]凝胶珠中,并在反应器中单独培养(以测试凝胶的影响),然后在有氧群落下进行培养。无氧和空气受限的条件。结果表明:(1)凝胶不影响颗粒的活性;(2)厌氧和需氧菌很容易固定在凝胶珠中,并在反应器中培养;(3)2,4的矿化作用,6-TCP(2,4,6-TCP负载量= 10-506 µmol / l /天),其降解的中间体和其他底物[葡萄糖+乙酸盐+丙酸酯(比例1:1:1)= COD负载如果严格控制培养参数[气流= 36–48 vvd(每天的空气量/反应器中的液体量),pH值为约7.5]。最终,凝胶在限流反应器的整个培养过程中(263天)没有保留其结构,但厌氧和有氧群落保持了活性并共同为矿化作用。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2001年第4期|524-530|共7页
  • 作者

    H. Gardin; J. Lebeault; A. Pauss;

  • 作者单位

    Département de Génie Chimique Université de Technologie de Compiègne BP 20-529 60205 Compiègne cedex France;

    Département de Génie Chimique Université de Technologie de Compiègne BP 20-529 60205 Compiègne cedex France;

    Département de Génie Chimique Université de Technologie de Compiègne BP 20-529 60205 Compiègne cedex France;

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