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首页> 外文期刊>Applied Microbiology and Biotechnology >κ-carrageenan/gelatin gel beads for the co-immobilization of aerobic and anaerobic microbial communities degrading 2,4,6-trichlorophenol under air-limited conditions
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κ-carrageenan/gelatin gel beads for the co-immobilization of aerobic and anaerobic microbial communities degrading 2,4,6-trichlorophenol under air-limited conditions

机译：κ-角叉菜胶/明胶凝胶珠用于在空气受限的条件下共固定降解2,4,6-三氯苯酚的好氧和厌氧微生物群落

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摘要

Alginate and κ-carrageenan gels were tested as bead materials for the co-immobilization of anaerobic and aerobic microorganisms for the mineralization of 2,4,6-trichlorophenol under air-limited conditions. Chemical, mechanical and thermal culture constraints were pre-defined and the gel resistances were established. Alginate was quickly eliminated because of its chemical instability in the culture media. In anaerobic conditions, the microorganisms transformed the substrates into CH4 and CO2. The κ-carrageenan gel did not enable these gases to diffuse. They remained as bubbles in the core of the beads and made the beads float. Gelatin was added to κ-carrageenan in order to change the carrier properties. No biogas bubbles appeared in the bead core during the cultures in anaerobic conditions and the beads reacted well to the culture conditions in the reactor. The co-immobilization of the anaerobic and aerobic microbial communities was successfully performed with the κ-carrageenan/gelatin gel (2% (w/w) of each polymer). The biological activities, measured by the impedancemetry technique, were preserved in the beads.
机译：测试了藻酸盐和κ-角叉菜胶凝胶作为在固定空气条件下共固定厌氧和好氧微生物矿化2,4,6-三氯苯酚的微珠材料。预定义了化学，机械和热培养的限制条件，并建立了抗凝胶性。由于藻酸盐在培养基中的化学不稳定，因此很快被淘汰。在厌氧条件下，微生物将底物转化为CH4 和CO2 。 κ-角叉菜胶不能使这些气体扩散。它们以气泡的形式留在珠子的核中，并使珠子漂浮。将明胶添加到κ-角叉菜胶中以改变载体性质。在厌氧条件下培养期间，珠芯中没有生物气泡出现，并且珠与反应器中的培养条件反应良好。用κ-角叉菜胶/明胶凝胶（每种聚合物的2％（w / w））成功地完成了厌氧和需氧微生物群落的共固定化。通过阻抗测定技术测量的生物活性保留在珠中。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2001年第4期|517-523|共7页
  • 作者

    H. Gardin; A. Pauss;

  • 作者单位

    Département de Génie Chimique Université de Technologie de Compiègne BP 20-529 60205 Compiègne cedex France;

    Département de Génie Chimique Université de Technologie de Compiègne BP 20-529 60205 Compiègne cedex France;

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