Mediated microbial biosensor using a novel yeast strain for wastewater BOD measurement

S. Trosok; B. Driscoll; J. Luong

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Mediated microbial biosensor using a novel yeast strain for wastewater BOD measurement

机译：新型酵母菌株介导的微生物生物传感器用于废水BOD的测量

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Two new yeast strains (SPT1 and SPT2) were isolated and immobilized on glassy carbon electrodes to form microbial biosensors for estimation of biochemical oxygen demand (BOD). Ferricyanide was proven to be the most efficient mediator to shuttle electrons from the redox center of reduced microbial enzymes to the electrode in the presence of excess glucose/glutamic acid (GGA). With a 3-fold greater metabolic assimilation capability and greater responses to various effluent samples, SPT1 was selected for sensor-BOD measurements. BOD estimations for the GGA standard resulted in an extended linear range: 2–100 mg/l. Response reproducibility was ±10% for a GGA standard containing 10 mg BOD/l. For analysis of pulp mill effluents, the BOD detection limit was 2 mg/l with a response time of 5 min.

机译：分离出两个新的酵母菌株（SPT1和SPT2），并将其固定在玻璃碳电极上，以形成微生物生物传感器，用于估算生化需氧量（BOD）。铁氰化物被证明是在过量葡萄糖/谷氨酸（GGA）存在下将电子从还原微生物酶的氧化还原中心传递到电极的最有效介质。 SPT1具有3倍更高的代谢同化能力和对各种流出物样品的更大响应，因此被选作传感器BOD测量。 GGA标准的BOD估计值导致线性范围扩大：2-100 mg / l。对于含10 mg BOD / l的GGA标准品，响应重现性为±10％。对于纸浆厂废水的分析，BOD检测限为2 mg / l，响应时间为5分钟。

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《Applied Microbiology and Biotechnology》 |2001年第4期|550-554|共5页
作者
S. Trosok; B. Driscoll; J. Luong;
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Biotechnology Research Institute National Research Council Canada 6100 Royalmount Avenue Montreal Quebec H4P 2R2 Canada;

Department of Natural Resource Sciences 21 111 Lakeshore Road Macdonald Campus McGill University Ste-Anne-de-Bellevue Quebec H9X 3V9 Canada;

Biotechnology Research Institute National Research Council Canada 6100 Royalmount Avenue Montreal Quebec H4P 2R2 Canada;

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Mediated microbial biosensor using a novel yeast strain for wastewater BOD measurement

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