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Changing the activities and structures of bovine serum albumin bound to graphene oxide

机译：改变牛血清白蛋白与氧化石墨烯结合的活性和结构

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摘要

To elucidate the binding mechanism of graphene oxide (GO) with bovine serum albumin (BSA) at molecular level, it is urgent to investigate their interactions in this work. The results indicated that multi noncovalent forces including hydrophobic force, hydrogen bonds, van der Waals and pi-pi* stacking interactions were involved in the adsorption forces of BSA on GO surface. The kinetics of BSA adsorption onto GO follows the pseudo-second-order kinetic model and was a fast process with high adsorption capacity. Some structure and functions of BSA were changed by GO binding. The decrease of melting temperature (T-m) and enthalpy changes (Delta H-m) implied that BSA on GO surface is more prone towards thermal denaturation. Drug competition experimental results showed that the binding affinities of BSA with 1-anilinonaphthalene-8-sulfonic acid (ANS) and ofloxacin (OFLX) were decreased to some extent. The esterase-like activity of BSA was obviously increased and BSA became more glycosylated in the presence of GO. Interestingly it was found that presence of GO significantly induced the conformation and activity changes of BSA. This work should help readers to understand and develop ideas for bio-related applications and to discuss the toxicological effects of graphene materials. (C) 2017 Elsevier B.V. All rights reserved.
机译：为了在分子水平上阐明氧化石墨烯（GO）与牛血清白蛋白（BSA）的结合机理，迫切需要研究它们之间的相互作用。结果表明，BSA在GO表面的吸附力涉及多个非共价力，包括疏水力，氢键，范德华力和pi-pi *堆积相互作用。 BSA吸附在GO上的动力学遵循伪二级动力学模型，是一个具有高吸附容量的快速过程。通过GO绑定更改了BSA的某些结构和功能。熔融温度（T-m）和焓变（ΔH-m）的降低表明GO表面的BSA更倾向于热变性。药物竞争实验结果表明，BSA与1-苯胺基萘-8-磺酸（ANS）和氧氟沙星（OFLX）的结合亲和力有所降低。在GO存在下，BSA的酯酶样活性明显增加，BSA变得更糖基化。有趣的是，发现GO的存在显着诱导了BSA的构象和活性变化。这项工作应帮助读者理解和发展生物相关应用的思想，并讨论石墨烯材料的毒理作用。 （C）2017 Elsevier B.V.保留所有权利。

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  • 来源
    《Applied Surface Science》 |2018年第ptaa期|1019-1029|共11页
  • 作者

    Zhang Hongmei; Zhu Zhaohua; Wang Yanqing; Fei Zhenghao; Cao Jian;

  • 作者单位

    Yancheng Teachers Univ, Jiangsu Key Lab Bioresources Saline Soils, Yancheng City 224002, Jiangsu, Peoples R China|Yancheng Teachers Univ, Inst Appl Chem & Environm Engn, Yancheng City 224002, Jiangsu, Peoples R China;

    Yancheng Teachers Univ, Inst Appl Chem & Environm Engn, Yancheng City 224002, Jiangsu, Peoples R China;

    Yancheng Teachers Univ, Jiangsu Key Lab Bioresources Saline Soils, Yancheng City 224002, Jiangsu, Peoples R China|Yancheng Teachers Univ, Inst Appl Chem & Environm Engn, Yancheng City 224002, Jiangsu, Peoples R China;

    Yancheng Teachers Univ, Jiangsu Key Lab Bioresources Saline Soils, Yancheng City 224002, Jiangsu, Peoples R China;

    Yancheng Teachers Univ, Inst Appl Chem & Environm Engn, Yancheng City 224002, Jiangsu, Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Graphene oxide; Bovine serum albumin; Binding mechanism; Conformational change; Activity;

    机译：氧化石墨烯;牛血清白蛋白;结合机理;构象变化;活性;

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