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Polyethylenimine surface layer for enhanced virus immobilization on cellulose

机译：聚乙烯亚胺表面层，用于增强病毒在纤维素上的固定

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摘要

Thin regenerated cellulose films are prepared by hydrolysis of cellulose acetate (CA). A polycation, namely polyethylenimine (PEI), is then adsorbed onto the films. From QCM-D analysis, PEI readily adsorbs from a 0.1% w/v solution in NaCl 0.2 M (ca. 100 ng cm(-2)). Further PEI adsorption steps at higher PEI concentrations induce a linear growth of the PEI films, suggesting that free adsorption sites still exist after the initial adsorption. The adsorbed PEI chains are resistant to variations of the ionic strength up to NaCl 1 M. Promisingly, the adsorption of T4D bacteriophages are 15-fold more efficient onto the PEI-treated, compared to the native regenerated cellulose films, as measured by QCM-D. This confirms the strong affinity between the negatively charged viruses and PEI, even at low PEI concentration, probably governed by strong electrostatic attractive interactions. This result explains the remarkable improvement of the affinity of medical masks for virus droplets when one of their cellulose layers was changed by two-PEI-functionalized cellulose-based filters. (C) 2016 Elsevier B.V. All rights reserved.
机译：再生纤维素薄膜很薄，是通过醋酸纤维素（CA）水解制备的。然后将聚阳离子，即聚乙烯亚胺（PEI）吸附到薄膜上。根据QCM-D分析，PEI容易从0.1％w / v的NaCl 0.2 M（约100 ng cm（-2））溶液中吸附。在更高的PEI浓度下，进一步的PEI吸附步骤会诱导PEI膜线性生长，这表明在初始吸附后仍然存在自由吸附位。吸附的PEI链可抵抗高达NaCl 1 M的离子强度变化。很可能地，与QCM- D．这证实了即使在低PEI浓度下，带负电荷的病毒与PEI之间也具有很强的亲和力，这很可能是由强的静电吸引力相互作用所决定的。该结果解释了当通过两个PEI功能化的纤维素基过滤器改变其纤维素层之一时，医用口罩对病毒滴的亲和力的显着改善。 （C）2016 Elsevier B.V.保留所有权利。

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  • 来源
    《Applied Surface Science》 |2016年第may1期|193-200|共8页
  • 作者

    Tiliket Ghania; Ladam Guy; Quang Trong Nguyen; Lebrun Laurent;

  • 作者单位

    Normandy Univ, Caen, France|UR CNRS, UMR 6270, Bd Maurice de Broglie, F-76821 Mont St Aignan, France|Lab Polymeres Biopolymeres & Surfaces, FR 3038, Bd Maurice de Broglie, F-76821 Mont St Aignan, France;

    Normandy Univ, Caen, France|Ctr Univ Evreux, La2B, EA MERCI 3829, 1 Rue 7eme Chasseurs,BP 281, F-27002 Evreux, France;

    Normandy Univ, Caen, France|UR CNRS, UMR 6270, Bd Maurice de Broglie, F-76821 Mont St Aignan, France|Lab Polymeres Biopolymeres & Surfaces, FR 3038, Bd Maurice de Broglie, F-76821 Mont St Aignan, France;

    Normandy Univ, Caen, France|UR CNRS, UMR 6270, Bd Maurice de Broglie, F-76821 Mont St Aignan, France|Lab Polymeres Biopolymeres & Surfaces, FR 3038, Bd Maurice de Broglie, F-76821 Mont St Aignan, France;

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  • 正文语种 eng
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  • 关键词

    Polyethylenimine; Bacteriophage adsorption; Polyelectrolyte adsorption; Cellulose film; QCM-D;

    机译：聚乙烯亚胺;噬菌体吸附;聚电解质吸附;纤维素膜;QCM-D;

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