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首页> 外文期刊>Applied Surface Science >Effects of scaffold surface morphology on cell adhesion and survival rate in vitreous cryopreservation of tenocyte-scaffold constructs
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Effects of scaffold surface morphology on cell adhesion and survival rate in vitreous cryopreservation of tenocyte-scaffold constructs

机译：支架表面形态对肌腱细胞支架构建体玻璃体冷冻保存中细胞粘附和存活率的影响

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摘要

The purpose of this study was to investigate the effects of scaffold surface morphology on cell adhesion and survival rate in vitreous cryopreservation of tenocyte-scaffold constructs. Tenocytes were obtained from tail tendons of rats. Polydimethylsiloxane (PDMS) was used to fabricate three types of scaffolds with varying surface morphological characteristics, i.e., smooth, micro-grooved, and porous surfaces, respectively. The tenocytes were seeded on the surfaces of the scaffolds to form tenocyte-scaffold constructs. The constructs were cryopreserved in a vitreous cryoprotectant (CPA) with a multi-step protocol. The cell adhesion to scaffolds was observed with electronic scanning microscopy (SEM). The elongation index of the living tenocytes and ratio of live/dead cell number were examined based on a live/dead dual fluorescent staining technique, and the survival rate of tenocytes was studied with flow cytometry (FC). The results showed the shapes of tenocytes varied between the different groups: flat or polygonal (on smooth surface), spindle (on micro-grooved surface), and spindle or ellipse (on porous surface). After thawing, the porous surface got the most living tenocytes and a higher survival rate, suggesting its potential application for vitreous cryopreservation of engineered tendon constructs. (C) 2016 Elsevier B.V. All rights reserved.
机译：本研究的目的是研究肌腱支架结构体在玻璃体冷冻保存中支架表面形态对细胞粘附和存活率的影响。从大鼠的尾腱获得肌腱细胞。聚二甲基硅氧烷（PDMS）被用于制造具有不同表面形态特征的三种类型的支架，即分别是光滑的，微沟槽的和多孔的表面。将肌腱细胞接种在支架的表面上以形成肌腱细胞支架构建体。使用多步骤方案将构建体冷冻保存在玻璃体防冻剂（CPA）中。用电子扫描显微镜（SEM）观察细胞对支架的粘附。基于活/死双重荧光染色技术，检测活肌腱细胞的伸长指数和活/死细胞数比，并通过流式细胞术（FC）研究了肌腱细胞的存活率。结果显示，肌腱细胞的形状在不同组之间变化：扁平或多边形（在光滑表面上），纺锤体（在微槽表面上）和纺锤体或椭圆形（在多孔表面上）。解冻后，多孔表面的肌腱细胞数量最多，存活率更高，表明其在工程性腱构建体玻璃体冷冻保存中的潜在应用。 （C）2016 Elsevier B.V.保留所有权利。

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  • 来源
    《Applied Surface Science》 |2016年第ptaa期|223-227|共5页
  • 作者

    Wang Zhi; Qing Quan; Chen Xi; Liu Cheng-Jun; Luo Jing-Cong; Hu Jin-Lian; Qin Ting-Wu;

  • 作者单位

    Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China|Luzhou Med Coll, Affiliated Hosp, Dept Bone & Joint Surg, Luzhou 646000, Peoples R China;

    Sichuan Coll Tradit Chinese Med, Mianyang 621000, Peoples R China|Sichuan Univ, West China Hosp, Regenerat Med Res Ctr, Chengdu 610041, Peoples R China;

    Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China;

    Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China;

    Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China;

    Hong Kong Polytech Univ, Inst Text & Clothing, Hong Kong, Hong Kong, Peoples R China;

    Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China;

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  • 正文语种 eng
  • 中图分类
  • 关键词

    Tenocyte; Scaffold; Surface morphology; Vitreous cryopreservation; Adhesion; Survival rate;

    机译：肌腱细胞;脚手架;表面形态;玻璃体冷冻保存;黏附;存活率;

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