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首页> 外文期刊>Applied Surface Science >Immobilization of cross linked Col-I-OPN bone matrix protein on aminolysed PCL surfaces enhances initial biocompatibility of human adipogenic mesenchymal stem cells (hADMSC)
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Immobilization of cross linked Col-I-OPN bone matrix protein on aminolysed PCL surfaces enhances initial biocompatibility of human adipogenic mesenchymal stem cells (hADMSC)

机译：交联的Col-I-OPN骨基质蛋白在氨基裂解的PCL表面上的固定可增强人脂肪形成间充质干细胞（hADMSC）的初始生物相容性

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摘要

In bone tissue engineering surface modification is considered as one of the important ways of fabricating successful biocompatible material. Addition of biologically active functionality on the surfaces has been tried for improving the overall biocompatibility of the system. In this study poly-ε-caprolactone film surfaces have been modified through aminolysis and immobilization process. Collagen type Ⅰ (COL-I) and osteopontin (OPN), which play an important role in osteogenesis, was immobilized onto PCL films followed by aminolysis treatment using 1,6-hexanediamine. Characterization of animolysed and immobilized surfaces were done by a number techniques using scanning electron microscopy (SEM), FT-IR, XPS, ninhydrin staining, SDS-PAGE and confocal microscopy and compared between the modified and un-modified surfaces. Results of the successive experiments showed that aminolysis treatment was homogeneously achieved which helped to entrap or immobilize Col-I-OPN proteins on surfaces of PCL film. In vitro studies with human adipogenic mesenchymal stem cells (Hadmsc) also confirmed the attachment and proliferation of cells was better in modified PCL surfaces than the unmodified surfaces. SEM, confocal microscopy and MTT assay showed a significant increase in cell spreading, attachment and proliferations on the biofunctionalized surfaces compared to the unmodified PCL surfaces at all-time points indicating the success of surface biofunctionalization.
机译：在骨组织工程中，表面修饰被认为是制造成功的生物相容性材料的重要方法之一。为了改善系统的整体生物相容性，已经尝试在表面上添加生物活性功能。在这项研究中，聚ε-己内酯薄膜表面已通过氨解和固定化过程进行了改性。在成骨中起重要作用的Ⅰ型胶原（COL-1）和骨桥蛋白（OPN）被固定在PCL膜上，然后用1,6-己二胺进行氨解处理。通过使用扫描电子显微镜（SEM），FT-IR，XPS，茚三酮染色，SDS-PAGE和共聚焦显微镜的多种技术对经过动画处理和固定的表面进行了表征，并在改性和未改性表面之间进行了比较。连续实验的结果表明，均一地实现了氨解处理，这有助于在PCL膜表面上捕获或固定Col-I-OPN蛋白。用人脂肪形成的间充质干细胞（Hadmsc）进行的体外研究还证实，修饰的PCL表面的细胞附着和增殖比未修饰的表面更好。 SEM，共聚焦显微镜和MTT分析显示，在所有时间点上，与未修饰的PCL表面相比，在生物功能化表面上的细胞扩散，附着和增殖显着增加，表明表面生物功能化的成功。

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  • 来源
    《Applied Surface Science》 |2014年第1期|97-106|共10页
  • 作者

    Young-Hee Kim; Anirban Jyoti; Ho-Yeon Song;

  • 作者单位

    Department of Microbiology, School of Medicine, Soonchunhyang University, Cheonan 330-090, Chungnum, South Korea;

    Department of Microbiology, School of Medicine, Soonchunhyang University, Cheonan 330-090, Chungnum, South Korea;

    Department of Microbiology, School of Medicine, Soonchunhyang University, Cheonan 330-090, Chungnum, South Korea;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Aminolysis; Poly-ε-caprolactone; Col-I-OPN; hADMSC; Biofunctionalization;

    机译：氨基分解;聚ε-己内酯;Col-I-OPN;hADMSC;生物功能化;

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