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首页> 外文期刊>Applied Surface Science >Protein adsorption and biomimetic mineralization behaviors of PLL-DNA multilayered films assembled onto titanium
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Protein adsorption and biomimetic mineralization behaviors of PLL-DNA multilayered films assembled onto titanium

机译：组装在钛上的PLL-DNA多层膜的蛋白质吸附和仿生矿化行为

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摘要

Titanium and its alloys are frequently used as surgical implants in load bearing situations, such as hip prostheses and dental implants, owing to their biocompatibility, mechanical and physical properties. In this paper, a layer-by-layer (LBL) self-assembly technique, based on the polyelectrolyte-mediated electrostatic adsorption of poly-L-lysine (PLL) and DNA, was used to the formation of multilayer on titanium surfaces. Then bovine serum albumin (BSA) adsorption and biomimetic mineralization of modified surfaces were studied. The chemical composition and wettability of assembled substrates were investigated by X-ray photoelectron spectroscopy (XPS), fluorescence microscopy and water contact angle measurement, respectively. The XPS analysis indicated that the layers were assembled successfully through electrostatic attractions. The measurement with ultraviolet (UV) spectrophotometer revealed that the LBL films enhanced ability of BSA adsorption onto titanium. The adsorption quantity of BSA on the surface terminated with PLL was higher than that of the surface terminated with DNA, and the samples of TiOH/P/D/P absorbed BSA most. Scanning electron microscopy (SEN!) and X-ray diffraction (XRD) showed that samples of assembled PLL or/and DNA had better bioactivity in inducing HA formation. Thus the assembling of PLL and DNA onto the surface of titanium in turn via a layer-by-layer self-assembly technology can improve the bioactivity of titanium.
机译：钛及其合金由于其生物相容性，机械和物理特性而经常在负重情况下用作外科植入物，例如髋关节假体和牙科植入物。在本文中，基于聚电解质介导的聚L-赖氨酸（PLL）和DNA的静电吸附的逐层（LBL）自组装技术被用于在钛表面上形成多层。然后研究了牛血清白蛋白（BSA）吸附和仿生矿化改性表面。分别通过X射线光电子能谱（XPS），荧光显微镜和水接触角测量研究了组装基板的化学成分和润湿性。 XPS分析表明，这些层通过静电引力成功组装。用紫外（UV）分光光度计的测量表明，LBL膜增强了BSA吸附在钛上的能力。 PLL终止的表面对BSA的吸附量高于DNA终止的表面对BSA的吸附量，并且TiOH / P / D / P样品吸收的BSA最多。扫描电子显微镜（SEN！）和X射线衍射（XRD）显示，组装的PLL或/和DNA样品在诱导HA形成中具有更好的生物活性。因此，通过逐层自组装技术依次将PLL和DNA组装到钛表面上，可以提高钛的生物活性。

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  • 来源
    《Applied Surface Science》 |2010年第2期|p.538-546|共9页
  • 作者

    Wenli Gao; Bo Feng; Yuxiang Ni; Yongli Yang; Xiong Lu; Jie Weng;

  • 作者单位

    Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering,Southwest Jiaotong University, Chengdu 610031, PR China;

    Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering,Southwest Jiaotong University, Chengdu 610031, PR China;

    Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering,Southwest Jiaotong University, Chengdu 610031, PR China;

    College of Material Science and Engineering, Sichuan University, Chengdu 610054, PR China;

    Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering,Southwest Jiaotong University, Chengdu 610031, PR China;

    Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering,Southwest Jiaotong University, Chengdu 610031, PR China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    layer-by-layer; poly-l-lysine; DNA; protein adsorption; biomimetic mineralization;

    机译：逐层聚赖氨酸脱氧核糖核酸;蛋白质吸附;仿生矿化;

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