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首页> 外文期刊>Applied Surface Science >Elastic modulus of suspended purple membrane measured by atomic force microscopy
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Elastic modulus of suspended purple membrane measured by atomic force microscopy

机译：原子力显微镜测量的悬浮紫色膜的弹性模量

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We have probed the mechanical properties of purple membrane (PM) in a physiological environment using the atomic force microscope (AFM). By suspending PM over nano-trenches, the elastic properties of PM can be evaluated free from the interaction with the substrate. Force-displacement curves were obtained on the suspended membrane and the data was compared to that of a simple model of a thin film over a trench. By fitting the data to the model, the elastic modulus of PM was estimated to be 8 MPa. When the membrane is repeatedly indented, we observed a change in the force-distance data consistent with damage to the two-dimensional crystal of PM. In this paper we demonstrate that the AFM allows us to evaluate the mechanics of biological membranes in their native conditions.
机译：我们已经使用原子力显微镜（AFM）在生理环境中探查了紫色膜（PM）的机械性能。通过将PM悬浮在纳米沟槽上，可以评估PM的弹性性能，而无需与基材相互作用。在悬浮膜上获得力-位移曲线，并将数据与沟槽上薄膜的简单模型的数据进行比较。通过将数据拟合到模型，PM的弹性模量估计为8 MPa。当膜被重复压入时，我们观察到力-距离数据的变化与PM二维晶体的损坏一致。在本文中，我们证明了AFM可以让我们评估其天然条件下生物膜的力学性能。

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