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首页> 外文期刊>Applied Surface Science >Applications Of Chitosan-based Thermo-sensitive Copolymers For Harvesting Living Cell Sheet
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Applications Of Chitosan-based Thermo-sensitive Copolymers For Harvesting Living Cell Sheet

机译：壳聚糖基热敏共聚物在收获活细胞片中的应用

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摘要

A thermo-sensitive chitosan-based copolymer hydrogel was used for harvesting living cell sheets. The hydrogel was tested for harvesting 3T3 cells after carrying out cell culture at 37 C and incubating the confluent cells at 20 C for spontaneous detachment of cell sheets from hydrogel surface without enzyme treatment. Results from cell viability assay and microscopy observations demonstrated that cells could attach to the hydrogel surface and maintain high viability and proliferation ability. Cell detachment efficiency from the hydrogel was about 80%. The detached cell sheet retained high viability and could proliferate again after transferred to a new culture surface.
机译：热敏壳聚糖基共聚物水凝胶用于收获活细胞片。在37℃下进行细胞培养并在20℃下孵育汇合的细胞以自发地将细胞片从水凝胶表面分离而不进行酶处理之后，测试水凝胶收获3T3细胞。细胞活力测定和显微镜观察的结果表明，细胞可以附着在水凝胶表面并保持高活力和增殖能力。从水凝胶的细胞分离效率为约80％。分离的细胞片保留了较高的生存能力，并且在转移到新的培养表面后可以再次增殖。

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