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首页> 外文期刊>Applied Surface Science >Cell Adhesive Ability Of A Biological Foam Ceramic With Surface Modification
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Cell Adhesive Ability Of A Biological Foam Ceramic With Surface Modification

机译：表面改性生物泡沫陶瓷的细胞黏附能力

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Biological foam ceramic is a promising material for tissue engineering scaffold because of its biocompatibility, biodegradation and adequate pores measured from micrometer to nanometers. The aim of this study was to evaluate the adhesion and proliferation of adipose-derived stromal cells (ADSCs) on the biological foam ceramic coated with fibronectin. ADSCs were harvested from SD rats and passaged three times prior to seeding onto biological foam surface modified with fibronectin (50 μg/ml). Scaffold without surface modification served as control. To characterize cellular attachment, cells were incubated on the scaffold for 1 h and 3 h and then the cells attached onto the scaffold were counted. The difference of proliferation was appraised using MTT assay at day 1. 3, 5 and 7 before the cells reached confluence. After 7 days of culture, scanning electron microscope (SEM) was chosen to assess cell morphology and attachment of ADSCs on the biological foam ceramic. Attachment of ADSCs on the biological foam ceramic surface modified with fibronectin at 1 h or 3 h was substantially greater than that in control. MTT assay revealed that ADSCs proliferation tendency of the experimental group was nearly parallel to that of control. SEM view showed that ADSCs in the experimental groups connected more tightly and excreted more collagen than that in control. The coating of fibronectin could improve the cell adhesive ability of biological foam ceramics without evident effect on proliferation.
机译：生物泡沫陶瓷由于其生物相容性，生物降解性以及从微米到纳米测量的足够的孔隙，因此是用于组织工程支架的有前途的材料。这项研究的目的是评估涂有纤连蛋白的生物泡沫陶瓷上脂肪来源的基质细胞（ADSCs）的粘附和增殖。从SD大鼠收获ADSC并传代3次，然后播种到用纤连蛋白（50μg/ ml）修饰的生物泡沫表面上。没有表面改性的支架用作对照。为了表征细胞附着，将细胞在支架上孵育1小时和3小时，然后计数附着在支架上的细胞。在细胞达到汇合之前，在第1、3、5和7天使用MTT测定法评估增殖的差异。培养7天后，选择扫描电子显微镜（SEM）评估细胞形态和ADSC在生物泡沫陶瓷上的附着。在1小时或3小时，ADSCs在用纤连蛋白修饰的生物泡沫陶瓷表面上的附着力明显大于对照。 MTT法检测结果表明，实验组ADSCs的增殖趋势与对照组接近。扫描电镜观察结果表明，实验组的ADSCs与对照组相比连接更紧密，分泌的胶原蛋白更多。纤连蛋白涂层可以提高生物泡沫陶瓷的细胞粘附能力，而对增殖没有明显影响。

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