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首页> 外文期刊>Applied Surface Science >The Cytochemistry Of Anaplastic Thyroid Tumour Cells And Differentiated Thyrocytes Analyzed By Tof-sims And Depth Profiling
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The Cytochemistry Of Anaplastic Thyroid Tumour Cells And Differentiated Thyrocytes Analyzed By Tof-sims And Depth Profiling

机译：Tof-sims和深度分析法分析间变性甲状腺肿瘤细胞和分化的甲状腺细胞的细胞化学

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The depth profiling procedure was utilized to compare the chemistry of anaplastic thyroid tumour cells SW1736 with that of primary cultured pig thyrocytes. Sputtering was performed with SF_5~+ and C_(60)~+ as primary ions and Bi_3~+ was used as primary ion for the analysis. The spectra from cells prior to sputtering, representing the cell surface chemistry, showed similar distribution patterns and intensity ratios. However, the peaks representing cholesterol signal and lipoxin were significantly higher in the tumour cell samples. The spectra from sputtered samples, representing intracellular chemistry, showed a significant decrease in the number of detectable peaks, especially after sputtering with SF_5~+. Sputtering conditions must be further developed to make the technique useful for detailed analysis of cells.
机译：深度分析程序用于比较间变性甲状腺肿瘤细胞SW1736与原代培养的猪甲状腺细胞的化学性质。以SF_5〜+和C_（60）〜+为主要离子，Bi_3〜+为主要离子进行溅射。溅射之前来自电池的光谱（代表电池表面化学性质）显示出相似的分布模式和强度比。然而，在肿瘤细胞样品中代表胆固醇信号和脂蛋白的峰明显更高。代表细胞内化学性质的溅射样品光谱显示出可检测峰的数量显着减少，尤其是在用SF_5〜+溅射后。必须进一步开发溅射条件，以使该技术可用于细胞的详细分析。

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