• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Surface Science >Gold Nanoparticle-enhanced Secondary Ion Mass Spectrometry And Its Bio-applications
【24h】

Gold Nanoparticle-enhanced Secondary Ion Mass Spectrometry And Its Bio-applications

机译：金纳米粒子增强二次离子质谱及其生物应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Enhancement of signals in time-of-flight secondary ion mass spectrometry (ToF-SIMS) studies is necessary to many biological applications. We have developed an efficient method of enhancing the signals of secondary ions from peptides using gold nanoparticles (AuNPs) attached to a well-controlled surface such as self-assembled monolayers (SAMs). AuNPs function as both signal enhancers and effective binding sites for peptides, which allow the high signal intensity from the peptides to produce well-contrasted ToF-SIMS images of peptides that are micropatterned on the surface of the AuNPs. For application, this AuNP-enhanced SIMS (NE-SIMS) provided the basis for the spectrum and images to assay protein kinases and their inhibitors. Phosphorylation efficiency and inhibitor effect were quantified by detecting mass change of the peptide substrates by kinase reaction. Maximum efficiency of phosphorylation was achieved from cysteine-tethered peptides with a spacer linker, indicating that accessibility of kinase was dependent on the surface orientation and length of the peptide substrate on the three-dimensional AuNPs. The activities of other enzymes such as phosphatase and protease could also be assayed using NE-SIMS. Our study shows that NE-SIMS can be applied as a useful tool for enzyme assay in biochip surfaces.
机译：飞行时间二次离子质谱（ToF-SIMS）研究中信号的增强对于许多生物学应用而言都是必需的。我们已经开发了一种有效的方法，可以使用附着在良好控制的表面（如自组装单分子膜（SAMs））上的金纳米颗粒（AuNP）增强来自肽的次级离子信号。 AuNPs既充当信号增强剂，又充当肽的有效结合位点，从而允许来自肽的高信号强度产生在AuNPs表面微图案化的对比度良好的ToF-SIMS图像。对于应用，这种AuNP增强的SIMS（NE-SIMS）为光谱和图像提供了基础，用于测定蛋白激酶及其抑制剂。通过通过激酶反应检测肽底物的质量变化来定量磷酸化效率和抑制剂作用。具有间隔子接头的半胱氨酸拴住的肽实现了最大的磷酸化效率，表明激酶的可及性取决于三维AuNPs上肽底物的表面方向和长度。其他酶（例如磷酸酶和蛋白酶）的活性也可以使用NE-SIMS分析。我们的研究表明，NE-SIMS可以用作生物芯片表面酶分析的有用工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号