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Silanization and antibody immobilization on SU-8

机译：SU-8的硅烷化和抗体固定化

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SU-8, an epoxy based negative photoresist, has emerged as a structural material for microfabricated sensors due to its attractive mechanical properties like low Young's modulus and chemical properties like inertness to various chemicals used in microfabrication. It can be used to fabricate MEMS structures of high aspect ratio. However, the use of SU-8 in BioMEMS application has been limited by the fact that immobilization of biomolecules on SU-8 surfaces has not been reported. In this study, the epoxy groups on the SU-8 surface were hydrolyzed in the presence of sulphochromic solution. Following this, the surface was treated with [3-(2-aminoethyl) aminopropyl]-trimethoxysilane (AEAPS). The silanized SU-8 surface was used to incubate human immunoglobulin (HIgG). The immobilization of HIgG was proved by allowing FITC tagged goat anti-human IgG to react with HIgG. This process of antibody immobilization was used to immobilize HIgG on microfabricated SU-8 cantilevers.
机译：SU-8是一种基于环氧的负性光致抗蚀剂，由于其有吸引力的机械性能（如低杨氏模量）和化学性能（如对微加工中使用的各种化学物质呈惰性）而已成为微加工传感器的结构材料。它可用于制造高纵横比的MEMS结构。然而，由于尚未报道过将生物分子固定在SU-8表面上的事实，限制了SU-8在BioMEMS应用中的使用。在这项研究中，SU-8表面上的环氧基在有色变色溶液的存在下被水解。之后，用[3-（2-氨基乙基）氨基丙基]-三甲氧基硅烷（AEAPS）处理表面。硅烷化的SU-8表面用于孵育人免疫球蛋白（HIgG）。通过允许FITC标记的山羊抗人IgG与HIgG反应来证明HIgG的固定化。该抗体固定过程用于将HIgG固定在微型SU-8悬臂上。

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