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首页> 外文期刊>Applied Surface Science >Cysteamine-modified diamond nanoparticles applied in cellular imaging and Hg~(2+) ions detection
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Cysteamine-modified diamond nanoparticles applied in cellular imaging and Hg~(2+) ions detection

机译：半胱胺修饰的金刚石纳米粒子在细胞成像和Hg〜（2+）离子检测中的应用

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摘要

Luminescent cysteamine-modified nanodiamond particles (ND-Cys) have been reported in surface-mediated energy transfer, cell imaging, and metal-ion recognition studies. In normalized photoluminescence (PL) spectral studies, the emission maxima of ND-Cys (at 50 mu g/mL in water) was fixed at 438 nm at 350 nm excitation, producing a blue emission with a quantum yield (Phi) of 0.13. In the dispersion range of 0-800 mu g/mL, ND-Cys exhibited agglomeration-induced energy transfer via red shifting of the PL peak from 438 nm to 451 nm. Analogous to PL studies, Raman interrogations also established the agglomeration and fixed the saturation limit to 500 mu g/mL in water. The low toxicity and biocompatibility of ND-Cys were demonstrated using methyl thiazolyl tetrazolium assay and time-dependent HeLa cell imaging. Subsequently, the Hg2+ selectivity by ND-Cys was revealed by an intense fluorescence peak shift from 440 nm to 463 nm. Fluorescence studies indicated that the detection limit of Hg2+ ions approximated 153 nM. Fourier-transform infrared and X-ray photoelectron spectroscopy (XPS) analyses supported the binding between free thiol (-SH) and amide (-C=O and -NH) groups of ND-Cys to Hg2+. The Hg2+-induced agglomeration and surface graphitization were successfully confirmed by X-ray powder diffraction, XPS, Raman, scanning electron microscopy, transmission electron microscopy, dynamic light scattering, and zeta potential analyses. Effective detection of Hg2+ ions by ND-Cys was validated through HeLa cell imaging at shortened time intervals.
机译：在表面介导的能量转移，细胞成像和金属离子识别研究中，已经报道了发光的半胱胺修饰的纳米金刚石颗粒（ND-Cys）。在归一化光致发光（PL）光谱研究中，ND-Cys的发射最大值（在水中50μg / mL）在350 nm激发下固定在438 nm，产生蓝色发射，量子产率（Phi）为0.13。在0-800μg / mL的分散范围内，ND-Cys通过PL峰从438 nm移至451 nm的红移而表现出团聚诱导的能量转移。与PL研究类似，拉曼审讯也确定了团聚作用，并将水中的饱和度限值固定为500μg / mL。使用甲基噻唑基四唑鎓测定法和时间依赖性HeLa细胞成像证明了ND-Cys的低毒性和生物相容性。随后，ND-Cys对Hg2 +的选择性通过从440 nm到463 nm的强烈荧光峰位移揭示。荧光研究表明，Hg2 +离子的检出限约为153 nM。傅立叶变换红外和X射线光电子能谱（XPS）分析支持ND-Cys的游离巯基（-SH）与酰胺基（-C = O和-NH）与Hg2 +的结合。通过X射线粉末衍射，XPS，拉曼，扫描电子显微镜，透射电子显微镜，动态光散射和zeta电位分析已成功证实了Hg2 +诱导的团聚和表面石墨化。通过HeLa细胞成像以较短的时间间隔验证了ND-Cys对Hg2 +离子的有效检测。

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