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首页> 外文期刊>Arabian Journal for Science and Engineering >Effect of Acidification on the Absorption and Fluorescence Properties of Sn(IV) Chlorin e6 Dichloride Trisodium Salt
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Effect of Acidification on the Absorption and Fluorescence Properties of Sn(IV) Chlorin e6 Dichloride Trisodium Salt

机译：酸化对Sn（IV）氯霉素e6二氯化三钠盐吸收和荧光性质的影响

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摘要

Chlorins are potential photosensitizers for photodynamic therapy because of their intense absorption in the red spectral region. In this study, the effect of pH on the absorption and fluorescence of Sn(IV) chlorin e6 dichloride trisodium salt was investigated. Absorption, fluorescence emission and fluorescence excitation spectra of the photosensitizer were recorded at different pH values in an analytical buffer solution with and without fetal bovine serum (FBS). In the neat buffer solution, changing the pH resulted in small shifts in both absorption and emission spectra. The shape of the excitation spectra also changed slightly, with shoulders almost vanishing below pH 5. Also, a pronounced reduction of the fluorescence intensity was observed upon acidification. The fluorescence intensity gradually decreased as the concentration of FBS was increased without shifting fluorescence maxima. The absorbance of the photosensitizer in different solvents was recorded and revealed that Sn(IV) chlorin e6 is highly soluble in polar solvents.
机译：氯霉素因在红色光谱区的强烈吸收而成为潜在的光动力疗法光敏剂。在这项研究中，研究了pH值对Sn（IV）二氢卟酚e6二氯化三钠盐的吸收和荧光的影响。在有和没有胎牛血清（FBS）的分析缓冲溶液中，在不同的pH值下记录了光敏剂的吸收，荧光发射和荧光激发光谱。在纯净的缓冲溶液中，改变pH值会导致吸收光谱和发射光谱发生小的变化。激发光谱的形状也略有变化，在pH低于5时肩几乎消失了。而且，酸化后观察到荧光强度明显降低。随着FBS浓度的增加，荧光强度逐渐降低，而荧光最大值没有改变。记录光敏剂在不同溶剂中的吸光度，并显示出Sn（IV）二氢卟酚e6在极性溶剂中高度可溶。

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