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首页> 外文期刊>Arabian Journal for Science and Engineering. Section A, Sciences >Assessment of competence of the Pseudomonas aeruginosa to solubilize insoluble form of zinc under various cultural parameters
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Assessment of competence of the Pseudomonas aeruginosa to solubilize insoluble form of zinc under various cultural parameters

机译：评估铜绿假单胞菌的竞争力，以在各种文化参数下溶解不溶性锌的溶解度

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摘要

Efficiency of Pseudomonas aeruginosa to solubilize insoluble form of zinc to soluble form was studied under various cultural parameters such as carbon (glucose, fructose, sucrose, maltose and lactose), nitrogen (ammonium sulphate, sodium nitrate, potassium nitrate and urea), pH (5.0, 7.0 and 9.0) and temperature (20, 30 and 40 °C). The experimental study was carried out by both qualitative (plate assay) and quantitative (broth assay) methods. The Zn-solubilizing efficiency of the isolatewas found more when glucosewas added as C-source in both plate (150%) and broth (16.62 mg/l) assays, and the efficiencywas less in the medium amended with sucrose as carbon source in both qualitative (105.8%) and quantitative (2.56mg/l) estimation . Among the four nitrogen sources, P. aeruginosa showed best solubilizing efficiency (262.5%) and solubilization of Zn (29.91 mg/l) in the presence of ammonium sulphate and recorded least solubilization efficiency in the presence of sodium nitrate as nitrogen source in both plate (181.82%) and broth (21.92 mg/l) assays. It showed highest solubilization of zinc from ZnO at the incubation temperature of 30 °C, and the activity was less at the temperature of 40 °C. pH 7 was the most favourable pH for solubilization, and the efficacywas least when the pHreached its alkalinity. Thus the study confirmed the efficiency of P. aeruginosa to solubilize insoluble form of zinc and can be used as a source of bioinoculants to eradicate zinc deficiency in plants.
机译：在各种文化参数（如碳（葡萄糖，果糖，蔗糖，麦芽糖和乳糖），氮（硫酸铵，硝酸钠，硝酸钾和尿素），研究了铜绿假单胞菌将不溶性锌的不溶形式溶于可溶性形式溶于可溶性形式的效率。（ 5.0,7.0和9.0）和温度（20,30和40°C）。通过定性（板测定）和定量（肉汤测定）方法进行实验研究。当在板（150％）和肉汤（16.62mg / L）测定中作为C源添加的葡萄糖加加油时，Zn增溶效率达到更多，并且在培养基中较少的效率较少，在定性中均为碳源。 （105.8％）和定量（2.56mg / l）估计。在四种氮源中，P.铜绿假单胞菌在硫酸铵存在下表现出最佳的溶解效率（262.5％）和Zn（29.91mg / L）的溶解，并在两个板中记录在硝酸钠作为氮源存在下的最低溶解效率（181.82％）和肉汤（21.92mg / L）测定。它在30℃温育温度下显示出来自ZnO的锌的最高溶解，并且在40℃的温度下，活性较少。 pH 7是溶解最有利的pH值，并且在对其碱度的碱度时最少的效率最小。因此，该研究证实了P.铜绿假单胞菌的效率溶解不溶形式的锌，可用作生物遗传群的来源，以消除植物缺锌。

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