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首页> 外文期刊>Archives of Animal Nutrition >Effects of keratinase supplementation of corn-soybean meal based diets on apparent ileal amino acid digestibility in growing pigs and serum amino acids, cytokines, immunoglobulin levels and loin muscle area in nursery pigs
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Effects of keratinase supplementation of corn-soybean meal based diets on apparent ileal amino acid digestibility in growing pigs and serum amino acids, cytokines, immunoglobulin levels and loin muscle area in nursery pigs

机译：补充玉米豆粕日粮中的角蛋白酶对生长猪表观回肠氨基酸消化率以及育成猪血清氨基酸，细胞因子，免疫球蛋白水平和腰部肌肉面积的影响

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摘要

Two experiments were conducted to evaluate effects of keratinase for growing and nursery pigs. In Exp. 1, six pigs (32.3 ± 2.8 kg body weight), fitted with a simple T-cannula at the distal ileum, were assigned to one of two 3 × 3 Latin squares involving three periods and three diets including a basal diet and the same diets supplemented with 0, 0.05 or 0.1% keratinase. Dietary keratinase supplementation increased the apparent ileal digestibility of crude protein (CP), arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, alanine, glutamic acid and proline (p < 0.05). Digestibility coefficients did not differ between pigs fed 0.05 and 0.1% keratinase. In Exp. 2, 24piglets weaned at 30 ± 2 d of age were used in a 2 × 2 factorial design experiment with two CP concentrations (19 vs. 22%) and two levels of keratinase supplementation (0 vs. 0.05%). Keratinase supplementation increased (p < 0.05) average daily gain, serum arginine concentration and loin muscle area but decreased (p < 0.05) serum interleukin-10 concentrations. The reduction in dietary CP level decreased (p < 0.05) serum urea nitrogen concentrations, isoleucine, serine and proline concentrations, but increased serum arginine concentrations. Few interactions between keratinase supplementation and dietary CP concentration were observed. This study indicated that dietary keratinase supplementation improved apparent ileal amino acid digestibility for growing pigs and had a positive effect on weight gain, immune response and loin muscle area for nursery pigs.
机译：进行了两个实验，以评估角蛋白酶对生长和保育猪的作用。在实验中1，六只猪（32.3±2.8公斤体重），在回肠末端装有一个简单的T型插管，被分配到两个3×3拉丁方格之一，涉及三个时期和三种饮食，包括基础饮食和补充0、0.05或0.1％角蛋白酶的相同饮食。饮食中的角蛋白酶补充增加了粗蛋白（CP），精氨酸，组氨酸，异亮氨酸，亮氨酸，赖氨酸，蛋氨酸，苯丙氨酸，苏氨酸，色氨酸，丙氨酸，谷氨酸和脯氨酸的表观回肠消化率（p <0.05）。饲喂0.05％和0.1％角蛋白酶的猪之间的消化系数没有差异。在实验中2，2断奶的24头仔猪在30±2 d的年龄用于2×2析因设计实验中的两个CP浓度（19 vs. 22％）和两个水平的角蛋白酶补充（0 vs. 0.05％）。角蛋白酶补充增加（p <0.05）平均每日增重，血清精氨酸浓度和腰部肌肉面积，但减少（p <0.05）血清白介素10浓度。饮食中CP水平降低降低（p <0.05）血清尿素氮浓度，异亮氨酸，丝氨酸和脯氨酸浓度，但增加了血清精氨酸浓度。补充角蛋白酶和饮食中CP浓度之间几乎没有相互作用。这项研究表明，日粮补充角蛋白酶可改善生长猪的表观回肠氨基酸消化率，并且对保育猪的体重增加，免疫反应和腰肌面积具有积极作用。

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