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首页> 外文期刊>Archives of Pharmacal Research >Anti-inflammatory changes of gene expression by Artemisia iwayomogi in the LPS-stimulated human gingival fibroblast: Microarray analysis
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Anti-inflammatory changes of gene expression by Artemisia iwayomogi in the LPS-stimulated human gingival fibroblast: Microarray analysis

机译：脂多糖刺激的人牙龈成纤维细胞中艾蒿的基因表达抗炎变化：微阵列分析

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摘要

The leaves and stems of Asteraceae Artemisia iwayomogi (Ai) for a long time have been known to inhibit inflammatory cytokine production and allergic reactions, and have been used to treat liver diseases. It needs to be elucidated in terms of global gene expression whether Ai has an influence as an anti-inflammatory agent on the cultured human gingival fibroblast stimulated with lipopolysaccharide (LPS). This study investigated the anti-inflammatory changes of the genes by Ai using the Affymetrix genechip human gene 1.0 ST array when the cultured human gingival fibroblast was treated with LPS. It was observed that the inflammation- and immune response-related genes were activated by LPS challenge in the cultured human gingival fibroblast. The array analysis showed that 65 of the 344 genes up-regulated by LPS stimulation, when compared to the control, were down-regulated by the Ai treatment. A number of inflammation- and immune response-related genes of the 65 genes were found. In addition, 78 of the 164 genes down-regulated by the LPS, when compared to the control, were up-regulated by the Ai treatment. The regulatory patterns of the representative genes were correlated with the real-time RT-PCR analysis. The Ai extract and its specific components, scopolin and scopoletin, significantly hindered the production of inflammatory mediators such as IL-6, TNF-α and nitrite in the LPS-challenged fibroblast. This study suggests that Ai can comprehensively inhibit the activation of the inflammation- and immune response-related genes and the inflammatory mediators in the human gingival fibroblast.
机译：长期以来，已知菊科艾叶蒿（Ai）的叶子和茎能够抑制炎症性细胞因子的产生和过敏反应，并已被用于治疗肝脏疾病。就全球基因表达而言，需要阐明Ai是否作为抗炎药对脂多糖（LPS）刺激的培养的人牙龈成纤维细胞有影响。本研究使用LPS处理培养的人牙龈成纤维细胞时，使用Affymetrix基因芯片人类基因1.0 ST阵列研究了Ai对基因的抗炎性变化。观察到，在培养的人牙龈成纤维细胞中，LPS激发激活了与炎症和免疫反应相关的基因。阵列分析显示，与对照相比，LPS刺激上调的344个基因中有65个被Ai处理下调。发现了65个基因中的许多与炎症和免疫反应相关的基因。此外，与对照相比，LPS下调的164个基因中有78个被Ai处理上调。代表性基因的调控模式与实时RT-PCR分析相关。 Ai提取物及其特定成分scopolin和scopoletin显着阻碍了LPS挑战的成纤维细胞中炎性介质的生成，例如IL-6，TNF-α和亚硝酸盐。这项研究表明，Ai可以全面抑制人类牙龈成纤维细胞中与炎症和免疫应答相关的基因以及炎症介质的激活。

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