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首页> 外文期刊>Archives of Pharmacal Research >Simultaneous determination of four marker components in Yukmijihwang Tang by high performance liquid chromatography/diode array detector
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Simultaneous determination of four marker components in Yukmijihwang Tang by high performance liquid chromatography/diode array detector

机译：高效液相色谱/二极管阵列检测器同时测定鱼米汤黄汤中的四种标记物

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摘要

Simultaneous determination method of four marker components, paeoniflorin, loganin, 5-hydroxymethyl-2-furaldehyde and paeonol in traditional herbal medicine, Yukmijihwang tang, was developed to achieve rapidly and systematic quality control by high performance liquid chromatography coupled with diode array detection. To develop this high performance liquid chromatography method, C18 column (5 μm, 120 Å, 4.6 mm × 150 mm) was used with gradient elution of water and methanol as mobile phase. Validation of the chromatography method was evaluated by linearity, recovery, and precision test. Calibration curves of standard components showed excellent linearity with R2 > 0.9990. Limits of detection and limits of quantification were in the ranges 0.01–0.02 and 0.04–0.07 μg/mL, respectively. The relative standard deviations of data of the intra-day and inter-day experiments were less than 1.15% and 3.76%, respectively. The results of recovery test were found to range from 94.88 to 107.43% with relative standard deviation values 0.07–2.66%. The results of validation suggested that this method was very accurate and stabilized.
机译：建立了同时测定传统草药中药材中药苷，loganin，5-羟甲基-2-呋喃醛和pa药中四种标记物成分的方法，以通过高效液相色谱结合二极管阵列检测实现快速，系统的质量控制。为了开发这种高效液相色谱方法，使用了C 18 色谱柱（5μm，120Å，4.6 mm×150 mm），以水和甲醇为梯度洗脱。通过线性，回收率和精密度测试评估色谱方法的有效性。标准成分的校准曲线具有良好的线性，R 2

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