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首页> 外文期刊>Archives of Toxicology >Study of the sensitising potential of various textile dyes using a biphasic murine local lymph node assay
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Study of the sensitising potential of various textile dyes using a biphasic murine local lymph node assay

机译：使用双相鼠局部淋巴结试验研究各种纺织品染料的敏化潜能

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Disperse dyes, which are suitable for dyeing synthetic fibres, are responsible for the great majority of allergic contact dermatitis (ACD) cases to textile dyes. The aim of the present study was to investigate the sensitising potential of various disperse dyes using a biphasic protocol of the local lymph node assay (LLNA). Briefly, mice were shaved over a surface of approximately 2 cm2 on their backs and treated using a “sensitisation-challenge protocol”. The shaved surface was treated once daily on days 1–3 with 50 μl of the test solution. Animals remained untreated on days 4–14. On days 15–17, mice were treated with 25 μl of the test solution on the dorsum of both ears. Mice were killed on day 19 with deep CO2 anaesthesia, the lymph nodes prepared and various end points, such as ear thickness, ear punch weight, lymph node weight, lymph node cell count and the proportion of various lymphocyte subpopulations, were determined by flow cytometry. The results were compared to control group treated with the vehicle alone. Our results showed that almost all of the tested textile dyes caused a significant increase in lymph node cell count and lymph node weight. We also observed an increase in ear thickness and ear punch weight in most of the concentrations tested for various textile dyes. We observed a decrease in CD4+ and CD8+ cells and an increase in CD19+, CD45+ and CD45+/1A+ cells in most of the cases, which is characteristic for allergens. The CD4+/CD69+ cells increased in only few experiments mainly with Disperse Blue 124 and Disperse Blue 106. Based on our results, the disperse dyes could be arranged in four groups on the basis of their sensitising potency in the following decreasing order (in parenthesis: lowest concentration causing a significant increase in lymph node cell number): group 1, strong: Disperse Blue 124 and Disperse Blue 106 (0.003%); group 2, moderate: Disperse Red 1 and Disperse Blue 1 (3%); group 3, weak: Disperse Orange 37 and Disperse Blue 35 (10%); and group 4, very weak: Disperse yellow 3 and Disperse Orange 3 (increase at 30% or no increase at 30%). In conclusion, our study shows that the biphasic LLNA protocol was proficient enough to study the sensitisation potential of tested textile dyes and provides data allowing to discriminate them according to their potency.
机译：分散染料适合纺织纤维的过敏性接触性皮炎（ACD）病例，这些染料适合对合成纤维进行染色。本研究的目的是使用局部淋巴结测定法（LLNA）的双相协议研究各种分散染料的敏化潜能。简而言之，将小鼠剃毛在其背部约2 cm 2 的表面上，并使用“敏化-挑战方案”进行处理。剃光的表面在第1-3天每天用50μl测试溶液处理一次。在第4-14天未治疗动物。在第15-17天，用两只耳朵背面的25μl测试溶液治疗小鼠。在第19天，用CO 2 深度麻醉杀死小鼠，准备好淋巴结，并确定各种终点，例如耳厚，耳穴重量，淋巴结重量，淋巴结细胞计数和各种比例通过流式细胞术确定淋巴细胞亚群。将结果与仅用媒介物治疗的对照组进行比较。我们的结果表明，几乎所有测试的纺织染料都会导致淋巴结细胞计数和淋巴结重量显着增加。我们还观察到，在各种纺织品染料测试的大多数浓度下，耳部厚度和耳模重量都会增加。在大多数情况下，我们观察到CD4 +和CD8 +细胞减少，而CD19 +，CD45 +和CD45 + / 1A +细胞增加，这是过敏原的特征。在仅使用分散蓝124和分散蓝106的少数实验中，CD4 + / CD69 +细胞有所增加。基于我们的结果，基于它们的增感染力，分散染料可以按下列递减顺序排列在四组中（括号中：最低浓度，导致淋巴结细胞数量显着增加）：第1组，强：分散蓝124和分散蓝106（0.003％）；第2组，中度：分散红1和分散蓝1（3％）；第3组，较弱：分散橙色37和分散蓝色35（10％）；第4组，非常弱：分散黄色3和分散橙色3（以30％的比例增加，或以30％的比例不增加）。总而言之，我们的研究表明，双相LLNA协议足以研究被测纺织品染料的敏化潜能，并提供了根据其效价区分它们的数据。

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