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首页> 外文期刊>Archives of Toxicology >Investigation of the sensitising and cross-sensitising potential of textile dyes and beta-lactam antibiotics using a biphasic mice local lymph node assay.
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Investigation of the sensitising and cross-sensitising potential of textile dyes and beta-lactam antibiotics using a biphasic mice local lymph node assay.

机译：使用双相小鼠局部淋巴结测定法研究纺织品染料和β-内酰胺抗生素的敏化和交叉敏化潜能。

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We used a modified protocol of the murine local lymph node assay (LLNA) to study the cross-sensitising potential of (a) textile dye disperse yellow 3 and its metabolite 2-amino-p-cresol, (b) two antibiotics, penicillin G and cefotiam. The test substances were applied in a biphasic manner, i.e. first on the shaved skin of the back followed by application on the dorsal side of the ears after 2 weeks. The end-points analysed included thickness and weight of an ear-biopsy, weight and cell number of the draining lymph node, and lymphocyte cell surface markers analysed by flow-cytometry. Disperse yellow 3 and its metabolite significantly altered the various end-points at both the tested concentrations (0.5 and 1%), thus demonstrating the sensitising potential of the two substances. The cross-sensitisation study showed significant modulation in the tested variables in the treated group as compared to the control, signifying cross-sensitisation potential of the two substances. Penicillin G and cefotiam showed significant changes in various end-points, pointing towards their sensitising potential. However, even at 50% concentration of the beta-lactams no significant change in any end-point indicating absence of cross-reactivity of the antibiotics was noticed. We conclude that a biphasic, modified protocol of the LLNA is a suitable approach to test for a cross-reactivity potential of two related compounds.
机译：我们使用鼠局部淋巴结测定法（LLNA）的改进方案来研究（a）纺织染料分散黄3及其代谢物2-氨基-对甲酚的交叉敏化潜力，（b）两种抗生素青霉素G和头孢替安。将测试物质以双相方式施用，即首先在背部的剃毛皮肤上施用，然后在两周后施用在耳朵的背侧。分析的终点包括耳部活检的厚度和重量，引流淋巴结的重量和细胞数，以及通过流式细胞术分析的淋巴细胞表面标志。分散的黄3及其代谢物在两种测试浓度（0.5和1％）下均显着改变了各种终点，因此证明了这两种物质的致敏潜力。交叉致敏研究表明，与对照组相比，治疗组的测试变量有明显的调节作用，表明这两种物质具有交叉致敏作用。青霉素G和头孢替安在各个端点均显示出显着变化，表明它们具有致敏作用。然而，即使在浓度为50％的β-内酰胺中，也没有发现任何明显变化，表明没有交叉反应性的抗生素。我们得出的结论是，LLNA的双相修改协议是一种测试两种相关化合物交叉反应潜力的合适方法。

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