• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Archives of Toxicology >The use of Fluoro-Jade in primary neuronal cell cultures
【24h】

The use of Fluoro-Jade in primary neuronal cell cultures

机译：荧光玉在原代神经元细胞培养中的应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fluoro-Jade (FJ) and its derivatives are widely used for histological staining of neurons undergoing neurodegeneration. With this dye, the entire structure of these neurons can be stained in a fast and reliable way in histopathological slices of the brain, with results comparable to those obtained with other methods such as the Nissle technique or silver staining. The question arose as to whether this method might be useful for in vitro neuronal cell cultures. Primary cortical neuronal cell cultures have been used as a sensitive and reliable system to detect compounds which induce neurodegenerative lesions (Schmuck et al. 2000). Additionally, various biochemical endpoints in this system allow the mode of action of these compounds to be identified. The target mechanism of FJ staining is unknown, and it may therefore be useful to compare FJ staining with one of the central endpoints in compound-induced neurodegeneration, interaction with the cytoskeleton as demonstrated by accumulations of neurofilaments (200 kD). Cortical neuronal cells were cultivated under standardized serum-free conditions. Once they had developed a stable network, the cells were treated with acrylamide, mipafox, diethyldithiocarbamate, glutamate, paraquat, paraoxon, and IDPN (ß,ß-imino dipropionitrile) for 7 days in the concentration range of 0.1–50 μg/ml. One half of the cell culture samples were tested directly after 7 days, the others were allowed to recover during a 7-day treatment-free period. Subsequently viability testing and quantification for FJ staining were performed. All compounds except paraoxon increased FJ staining after 7 days, and this signal increased slightly during the recovery period with glutamate and acrylamide. With mipafox and IDPN the signal decreased slightly. Paraoxon increased FJ staining only after the recovery period. The intensity of FJ staining did not always correlate with neurofilament destruction or cytotoxicity. It can therefore be assumed that FJ targets a different cellular endpoint. Interestingly, paraoxon, a compound which does not induce neurodegeneration, increased FJ staining only in the recovery phase; this pointed to a neurotoxic mechanism which sets it apart from the other model compounds.
机译：氟玉石（FJ）及其衍生物广泛用于神经变性的神经元的组织学染色。使用这种染料，可以在大脑的组织病理切片中以快速，可靠的方式对这些神经元的整个结构进行染色，其结果可与采用其他方法（如Nissle技术或银染）获得的结果相媲美。产生了关于该方法对于体外神经元细胞培养是否有用的问题。原代皮层神经元细胞培养已被用作检测诱导神经退行性病变的化合物的灵敏可靠的系统（Schmuck et al。2000）。另外，该系统中的各种生化终点允许鉴定这些化合物的作用方式。 FJ染色的靶机制尚不清楚，因此，将FJ染色与化合物诱导的神经变性，与细胞骨架的相互作用中的中心终点之一进行比较可能是有用的，如神经丝的堆积（200 kD）所示。在标准的无血清条件下培养皮质神经元细胞。一旦它们建立了稳定的网络，就用0.1–50μg/ ml的浓度的丙烯酰胺，mapafox，二乙基二硫代氨基甲酸酯，谷氨酸，百草枯，对氧磷和IDPN（ß，ß-亚氨基二丙腈）处理细胞7天。 7天后直接测试了一半的细胞培养物样品，其余的则允许在7天的无治疗期内恢复。随后进行活力测试和FJ染色定量。除对氧磷外，所有化合物在7天后均会增加FJ染色，并且在谷氨酸和丙烯酰胺的恢复期间，该信号略有增加。使用mipafox和IDPN时，信号略有下降。对氧磷仅在恢复期后才增加FJ染色。 FJ染色的强度并不总是与神经丝破坏或细胞毒性有关。因此，可以假设FJ靶向不同的细胞端点。有趣的是，对氧磷是一种不会引起神经变性的化合物，仅在恢复期才增加FJ染色。这指出了一种神经毒性机制，使其与其他模型化合物区分开来。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号