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首页> 外文期刊>Arthritis & Rheumatism >The response of human anulus fibrosus cells to cyclic tensile strain is frequency-dependent and altered with disc degeneration
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The response of human anulus fibrosus cells to cyclic tensile strain is frequency-dependent and altered with disc degeneration

机译：人环纤维细胞对循环拉伸应变的反应是频率依赖性的，并随着椎间盘退变而改变

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ObjectiveMechanical loads are important for homeostasis of the intervertebral disc (IVD) cell matrix, with physiologic and nonphysiologic loads leading to matrix anabolism and catabolism, respectively. Previous investigations into the effects of load on disc cells have predominantly used animal models, with the limited number of human studies focusing primarily on nucleus pulposus cells. The aim of this study was to examine the effect of cyclic tensile strain (CTS) on human anulus fibrosus (AF) cells to ascertain whether the response was frequency-dependent and to compare AF cells derived from nondegenerated and degenerated tissue samples.MethodsAF cells were isolated from nondegenerated and degenerated human IVDs, expanded in monolayer, and cyclically strained for 20 minutes, applying 10% strain at a frequency of 1.0 Hz or 0.33 Hz with the use of a Flexcell strain device. Total RNA was extracted from the cells at baseline (control) and at 1, 3, and 24 hours following application of CTS. Real-time quantitative polymerase chain reaction was used to analyze gene expression of matrix proteins (aggrecan, type I collagen, and type II collagen) and enzymes (matrix metalloproteinases [MMPs] 3, 9, 13, and ADAMTS-4).ResultsThe expression of catabolic genes (MMP-3 and ADAMTS-4) in AF cells derived from nondegenerated tissue decreased in response to 1.0 Hz of CTS, whereas changing the frequency to 0.33 Hz resulted in a shift toward matrix catabolism. Application of 1.0 Hz of CTS reduced anabolic gene expression (aggrecan and type I collagen) in AF cells derived from degenerated tissue, with 0.33 Hz of CTS resulting in increased catabolic gene expression.ConclusionThe response of human AF cells to CTS is frequency-dependent and is altered by degeneration.
机译：目的机械负荷对于椎间盘（IVD）细胞基质的稳态至关重要，生理和非生理负荷分别导致基质合成代谢和分解代谢。先前对负荷对椎间盘细胞的影响的研究主要使用动物模型，而有限数量的人类研究主要集中于髓核细胞。这项研究的目的是研究循环拉伸应变（CTS）对人环纤维（AF）细胞的影响，以确定反应是否是频率依赖性的，并比较衍生自未变性和变性组织样品的AF细胞。分离自未变性和变性的人IVD，单层扩增并循环拉伸20分钟，使用Flexcell应变装置以1.0 Hz或0.33 Hz的频率施加10％的应变。在应用CTS后的基线（对照），1、3和24小时从细胞中提取总RNA。实时定量聚合酶链反应用于分析基质蛋白（aggrecan，I型胶原和II型胶原）和酶（基质金属蛋白酶[MMP] 3、9、13和ADAMTS-4）的基因表达。非变性组织衍生的AF细胞中分解代谢基因（MMP-3和ADAMTS-4）的表达响应于1.0 Hz的CTS而降低，而将频率更改为0.33 Hz导致向基质分解代谢的转变。施加1.0 Hz的CTS可降低变性组织衍生的AF细胞中合成代谢基因的表达（聚集蛋白聚糖和I型胶原），而0.33 Hz的CTS则可导致分解代谢基因的表达增加。被变性改变。

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