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首页> 外文期刊>Artificial Organs >Pulsatile Culture of a Poly(DL-Lactic-Co-Glycolic Acid) Sandwiched Cell/Hydrogel Construct Fabricated Using a Step-by-Step Mold/Extraction Method
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Pulsatile Culture of a Poly(DL-Lactic-Co-Glycolic Acid) Sandwiched Cell/Hydrogel Construct Fabricated Using a Step-by-Step Mold/Extraction Method

机译：使用逐步成型/提取方法制造的聚（DL-乳酸-乙醇酸）夹心细胞/水凝胶构建体的脉冲培养

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摘要

To overcome the weak mechanical properties of cell/hydrogel composites, a poly(DL-lactic-co-glycolic acid) sandwiched adipose-derived stem cell (ADSC)/fibrin construct was fabricated using a step-by-step mold/extraction method to generate the middle smooth muscle layer of natural blood vessels. A pulse bioreactor with an adjustable 0–0.2?MPa pressure, 0–7% pulse amplitude, and 0–80 times/min pulse frequency was developed to mimic the liquid movement in the natural blood vessels. This new type of pulse bioreactor is sterilizable and dismantles easily. A comparative study was conducted with static and dynamic in vitro cultures. Exogenous growth factors, such as hepatocyte growth factor, platelet-derived growth factor BB, transforming growth factor β1, and basic fibroblast growth factor were used as additives in the culture medium for inducing the ADSCs into smooth muscle cells. The dynamic training, integrated with the growth factor, induced the transformation of ADSCs into smooth muscle-like cells with regular arrangement. This strategy shows promise of being widely used in tissue engineering and complex organ manufacturing.
机译：为了克服细胞/水凝胶复合材料的弱机械性能，使用分步模具/提取方法制备了聚（DL-乳酸-乙醇酸）夹心的脂肪衍生干细胞（ADSC）/纤维蛋白结构。产生天然血管的中间平滑肌层。开发了可调节压力0-0.2？MPa，脉冲幅度0-7％，脉冲频率0-80次/分钟的脉冲生物反应器，以模仿天然血管中的液体运动。这种新型脉冲生物反应器可消毒且易于拆卸。进行了静态和动态体外培养的比较研究。肝细胞生长因子，血小板衍生生长因子BB，转化生长因子β1和碱性成纤维细胞生长因子等外源性生长因子被用作培养基中的添加剂，以诱导ADSCs进入平滑肌细胞。动态训练与生长因子结合，诱导ADSCs转化为规则排列的平滑肌样细胞。该策略显示出有望在组织工程和复杂器官制造中广泛使用的前景。

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