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首页> 外文期刊>Artificial Organs >Cellulose Acetate Beads Activate the Complement System but Inactivate the Anaphylatoxins Generated
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Cellulose Acetate Beads Activate the Complement System but Inactivate the Anaphylatoxins Generated

机译：醋酸纤维微珠激活补体系统，但灭活产生的过敏毒素

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The generation of anaphylatoxins, particularly C5a, is important in extracorporeal circulation therapies such as granulocyte/monocyte apheresis, which activates the complement system and elevates C5a levels. However, no side effects of granulocyte/monocyte apheresis using cellulose acetate beads have been reported. To investigate the mechanism of complement activation, we prepared plasma from cellulose acetate bead-treated blood (P-CAB) and compared it with zymosan-activated plasma (ZAP). Anaphylaxis activity was measured by skin test, and the activity of carboxypeptidase, which inactivates C5a, was measured by colorimetric assay. Pro-carboxypeptidase R and neutrophil elastase concentrations were measured by enzyme-linked immunosorbent assay. Although C5a was generated in P-CAB, the anaphylaxis activity of P-CAB was lower than that of ZAP. Carboxypeptidase activity and pro-carboxypeptidase R levels were suppressed in P-CAB, but not in ZAP. Furthermore, neutrophil elastase levels increased in P-CAB. The decreases in carboxypeptidase activity and inactivation of anaphylatoxin were inhibited by a neutrophil elastase inhibitor. These results suggest that cellulose acetate beads initiate the activation of carboxypeptidase R via elastase release, thereby inducing the inactivation of anaphylatoxin.
机译：过敏毒素的产生，特别是C5a，在体外循环疗法（如粒细胞/单核细胞单采血液分离）中很重要，它激活补体系统并提高C5a的水平。然而，没有报道使用乙酸纤维素珠粒的粒细胞/单核细胞单采的副作用。为了研究补体激活的机制，我们从醋酸纤维素珠处理的血液（P-CAB）中制备血浆，并将其与酵母聚糖激活的血浆（ZAP）进行比较。通过皮肤测试测量过敏反应活性，并且通过比色分析测量灭活C5a的羧肽酶的活性。通过酶联免疫吸附测定法测定羧肽酶原R和中性粒细胞弹性蛋白酶的浓度。尽管在P-CAB中产生了C5a，但是P-CAB的过敏反应活性低于ZAP。羧肽酶活性和羧肽酶原R水平在P-CAB中被抑制，但在ZAP中未受到抑制。此外，P-CAB中的中性粒细胞弹性蛋白酶水平增加。嗜中性粒细胞弹性蛋白酶抑制剂抑制了羧肽酶活性的降低和过敏毒素的失活。这些结果表明，乙酸纤维素珠粒通过弹性蛋白酶的释放而引发羧肽酶R的活化，从而诱导了过敏毒素的失活。

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