...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Asian Journal of Andrology >Expression and localization of CKLFSF2 in human spermato-genesis
【24h】

Expression and localization of CKLFSF2 in human spermato-genesis

机译：CKLFSF2在人类精子发生中的表达和定位

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aim: To investigate the expression and subcellular localization of chemokine-like factor superfamily 2 (CKLFSF2) in human testis and its potential role in spermatogenesis. Methods: A specific polyclonal antibody against CKLFSF2 was raised. The expression and cellular localization of CKLFSF2 in the seminiferous tubules was checked by immu-nohistochemistry method. Also, in situ hybridization was applied to localize the mRNA distribution. The EGFP-CKLFSF2 fusion protein was expressed in COS-7 cells to localize its subcellular location in vitro. In addition, the abnormal expression of CKLFSF2 in testes of patients with male infertility was assayed by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry methods. Results: Having a close correlation with spermatogenesis defects, CKLFSF2 was specifically expressed in meiotic and post-meiotic germ cells, which were localized to the endoplasmic reticulum (ER) near the Golgi apparatus. Conclusion: CKLFSF2 could play important roles in the process of meiosis and spermiogenesis, and might be involved in the vesicular transport or membrane apposition events in the endoplasmic reticulum.
机译：目的：探讨趋化因子样因子超家族2（CKLFSF2）在人睾丸中的表达和亚细胞定位及其在精子发生中的潜在作用。方法：制备针对CKLFSF2的特异性多克隆抗体。用免疫组织化学方法检测CKLFSF2在曲细精管中的表达和细胞定位。同样，应用原位杂交来定位mRNA分布。 EGFP-CKLFSF2融合蛋白在COS-7细胞中表达以在体外定位其亚细胞位置。此外，通过逆转录聚合酶链反应（RT-PCR）和免疫组化方法检测了男性不育患者睾丸中CKLFSF2的异常表达。结果：CKLFSF2与精子发生缺陷密切相关，在减数分裂和减数分裂后的生殖细胞中特异性表达，它们定位于高尔基体附近的内质网（ER）。结论：CKLFSF2可能在减数分裂和精子发生过程中起重要作用，并可能参与内质网的囊泡转运或膜并置事件。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号