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首页> 外文期刊>Asian Journal of Andrology >Porcine vesical acellular matrix graft of tunica albuginea for penile reconstruction
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Porcine vesical acellular matrix graft of tunica albuginea for penile reconstruction

机译：猪白膜囊泡脱细胞基质移植物的阴茎重建

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Aim: To characterize the feasibility of the surgical replacement of the penile tunica albuginea (TA) and to evaluate the value of a porcine bladder acellular matrix (BAM) graft. Methods: Acellular matrices were constructed from pigs' bladders by cell lysis, and then examined by scanning electron microscopy (SEM). Expression levels of the mRNA of the vascular endothelial growth factor (VEGF) receptor, fibroblast growth factor (FGF)-1 receptor, neuregulin, and brain-derived neurotrophic factor (BDNF) in the acellular matrix and submucosa of the pigs' bladders were determined through the reverse transcription-polymerase chain reaction (PCR). A 5 mm x 5 mm square was excised from the penile TA of nine rabbits. The defective TA was then covered in porcine BAM. Equal numbers of animals were sacrificed and histochemically examined at 2, 4 and 6 months after implantation. Results: SEM of the BAM showed collagen fibers with many pores. VEGF receptor, FGF-1 receptor and neuregulin mRNA were expressed in the porcine BAM; BDNF mRNA was not detected. Two months after implantation, the graft sites exhibited excellent healing without contracture, and the fusion between the graft and the neighboring normal TA appeared to be well established. There were no significant histological differences between the implanted tunica and the normal control tunica at 6 months after implantation. Conclusion: The porcine BAM graft resulted in a structure which was sufficiently like that of the normal TA. This implantation might be considered applicable to the reconstruction of the TA in conditions such as trauma or Peyronie's disease.
机译：目的：描述外科手术替代阴茎白膜（TA）的可行性，并评估猪膀胱脱细胞基质（BAM）移植物的价值。方法：通过细胞裂解从猪的膀胱中构建脱细胞基质，然后通过扫描电子显微镜（SEM）进行检查。测定猪膀胱无细胞基质和粘膜下层中血管内皮生长因子（VEGF）受体，成纤维细胞生长因子（FGF）-1受体，神经调节蛋白和脑源性神经营养因子（BDNF）mRNA的表达水平通过逆转录聚合酶链反应（PCR）。从9只兔子的阴茎TA切出5mm×5mm见方。然后用猪BAM覆盖有缺陷的TA。植入后2、4和6个月，处死相等数量的动物并进行组织化学检查。结果：BAM的SEM显示胶原纤维具有许多孔。猪BAM中表达VEGF受体，FGF-1受体和神经调节蛋白mRNA。未检测到BDNF mRNA。植入后两个月，移植物部位表现出优异的愈合能力，没有挛缩，并且移植物与邻近的正常TA之间的融合似乎已经确立。植入后6个月，被膜与正常对照膜在组织学上无明显差异。结论：猪BAM移植物的结构与正常TA十分相似。这种植入可能被认为适用于在诸如创伤或佩罗尼氏病的情况下重建TA。

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