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首页> 外文期刊>Asian Journal of Andrology >Influence of selenium induced oxidative stress on spermatogenesis and lactate dehydrogenase-X in mice testis
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Influence of selenium induced oxidative stress on spermatogenesis and lactate dehydrogenase-X in mice testis

机译：硒诱导的氧化应激对小鼠睾丸生精和乳酸脱氢酶-X的影响

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Aim: To evaluate the effect of oxidative stress on the spermatogenesis and lactate dehydrogenase-X (LDH-X) activity in mouse testis. Methods: For creating different levels of oxidative stress in mice, three selenium (Se) level diets were fed in separate groups for 8 weeks. Group 1 animals were fed yeast-based Se-deficient (0.02 ppm) diet. Group 2 and Group 3 animals were fed with the same diet supplemented with 0.2 ppm and 1 ppm Se as sodium selenite, respectively. After 8 weeks, biochemical and histopathological observations of the testis were carried out. LDH-X levels in the testis were analyzed by western immunoblot and ELISA. Results: A significant decrease in testis Se level was observed in Group 1 animals, whereas it was enhanced in Group 3 as compared to Group 2. The glutathione peroxidase (GSH-Px) activity was significantly reduced in both the liver and testis in Group 1, but not in Group 2 and 3. A significant increase in the testis glutathione-S-transferase (GST) activity was observed in Group 1, whereas no significant change was seen in Groups 2 and 3. Histological analysis of testis revealed a normal structure in Group 2. A significant decrease in the germ cell population in Group 1 was observed as compared to Group 2 with the spermatids and mature sperm affected the most. Decrease in the lumen size was also observed. In the Se-excess group (Group 3), displacement of germ cell population was observed. Further, a decrease in the LDH-X level in testis was observed in Group 1. Conclusion: Excessive oxidative stress in the Se deficient group, as indicated by changes in the GSH-Px/GST activity, affects the spermatogenic process with a reduction in mature sperm and in turn the LDH-X level.
机译：目的：评估氧化应激对小鼠睾丸精子发生和乳酸脱氢酶-X（LDH-X）活性的影响。方法：为了在小鼠中产生不同水平的氧化应激，将3种硒（Se）水平的饮食分批喂养8周。第1组动物接受基于酵母的硒缺乏（0.02 ppm）饮食。第2组和第3组动物分别饲喂添加了0.2 ppm和1 ppm Se的亚硒酸钠的相同饮食。 8周后，进行睾丸的生化和组织病理学观察。用Western免疫印迹和ELISA分析睾丸中LDH-X的水平。结果：在第1组动物中观察到睾丸Se水平显着下降，而在第3组中则比第2组增加。在第1组中肝脏和睾丸中的谷胱甘肽过氧化物酶（GSH-Px）活性均显着降低。 ，但在第2和3组中则没有。在第1组中观察到睾丸谷胱甘肽S-转移酶（GST）活性显着增加，而在第2和3组中未见明显变化。睾丸的组织学分析显示其结构正常在第2组中，与第2组相比，第1组的生殖细胞数量显着减少，其中精子和成熟精子受影响最大。还观察到管腔尺寸减小。在硒过多组（第3组）中，观察到生殖细胞数量的移位。此外，在第1组中，睾丸中的LDH-X水平下降。结论：如GSH-Px / GST活性的变化所示，Se缺乏症组中的过度氧化应激会影响精子形成过程，降低GSH-Px / GST活性。成熟的精子，然后是LDH-X水平。

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