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首页> 外文期刊>Asian journal of water, environment and pollution >Molecular Detection of Group C Rotavirus in Environmental Samples in Giza, Egypt
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Molecular Detection of Group C Rotavirus in Environmental Samples in Giza, Egypt

机译：埃及吉萨市环境样品中C组轮状病毒的分子检测

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摘要

Group C rotavirus (RVC) has been identified as important enteric pathogen associated with acute gastroenteritis in human, especially in outbreaks. To evaluate the presence of RVC in environmental samples, one-year study was performed in Giza, Egypt. A total of 64 sewage (raw and treated) with 32 sewage sludge samples from Zenin wastewater treatment plant (WWTP) and 72 drainage water with 24 sediment samples from El-Rahawy drain which receives the sewage effluent of Zenin WWTP were collected. After concentration of the collected samples, semi-nested RT-PCR using specific primers was used for amplification of the RVC VP7 gene. The overall detection rates were 21.9% (7 of 32) for the raw sewage, 6% (2 of 32) for the treated sewage, 12.5% (4 of 32) for the sewage sludge, 22% (16 of 72) for the drainage water, and 16.6% (4 of 24) for the drainage sediment. In addition, the virus was abundant in cold months (October-March) and less prevalent in hot months (April-September). The widespread occurrence of RVC in this study reflects that RVC circulates at a relatively high frequency in the Egyptian population.
机译：C组轮状病毒（RVC）已被确定为与人类急性胃肠炎相关的重要肠道病原体，尤其是在暴发中。为了评估环境样品中RVC的存在，在埃及吉萨进行了为期一年的研究。总共收集了来自Zenin污水处理厂（WWTP）的32个污水污泥样品的64个污水（原始和处理过的）和来自El-Rahawy排水的72个污水和24个沉积物样品的污水，这些污水接收了Zenin WWTP的污水。浓缩收集的样品后，使用特定引物的半巢式RT-PCR扩增RVC VP7基因。总体检出率为：原污水为21.9％（32之7），处理后污水为6％（32之2），污水污泥为12.5％（32之4），污水处理厂为22％（72之16）。排水，其中16.6％（24之4）用于排水。此外，该病毒在寒冷月份（10月至3月）丰富，而在炎热月份（4月至9月）流行。在这项研究中，RVC的广泛出现反映了RVC在埃及人口中的传播频率相对较高。

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