Photochemistry on the Space Station-Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station

Coussot Gaelle; Le Postollec Aurelie; Incerti Sebastien; Baque Mickael; Faye Clement; Vandenabeele-Trambouze Odile; Cottin Herve; Ravelet Corinne; Peyrin Eric; Fiore Emmanuelle; Vigier Flavie; Caron Jerome; Chaput Didier; Przybyla Bartos; Berger Thomas; Dobrijevic Michel

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Photochemistry on the Space Station-Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station

机译：空间站上的光化学-适体对空间条件的抵抗力：辐照设施中的颗粒暴露和国际空间站外的实际暴露

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Some microarray-based instruments that use bioaffinity receptors such as antibodies or aptamers are under development to detect signatures of past or present life on planetary bodies. Studying the resistance of such instruments against space constraints and cosmic rays in particular is a prerequisite. We used several ground-based facilities to study the resistance of aptamers to various types of particles (protons, electrons, neutrons, and carbon ions) at different energies and fluences. We also tested the resistance of aptamers during the EXPOSE-R2 mission outside the International Space Station (ISS). The accumulated dose measured after the 588 days of this mission (220 mGy) corresponds to the accumulated dose that can be expected during a mission to Mars. We found that the recognition ability of fluorescently labeled aptamers was not significantly affected during short-term exposure experiments taking into account only one type of radiation at a time. However, we demonstrated that the same fluorescent dye was significantly affected by temperature variations (-21 degrees C to +58 degrees C) and storage throughout the entirety of the ISS experiment (60% of signal loss). This induced a large variability of aptamer signal in our analysis. However, we found that 50% of aptamers were still functional after the whole EXPOSE-R2 mission. We conclude that aptamer-based instruments are well suited for in situ analysis on planetary bodies, but the detection step requires additional investigations.

机译：一些使用生物亲和受体（例如抗体或适体）的基于微阵列的仪器正在开发中，以检测行星体过去或现在的生命特征。先决条件是研究此类仪器对空间限制，尤其是宇宙射线的抵抗力。我们使用了几种基于地面的设施来研究适体在不同能量和密度下对各种类型的粒子（质子，电子，中子和碳离子）的抵抗力。我们还在国际空间站（ISS）以外的EXPOSE-R2任务期间测试了适体的抵抗力。在执行此任务的588天后测量的累积剂量（220 mGy）对应于在执行火星任务期间可以预期的累积剂量。我们发现在荧光暴露的适体的识别能力在短期暴露实验中并没有受到显着影响，因为一次只考虑一种辐射。但是，我们证明了相同的荧光染料在整个ISS实验的整个过程（温度损失为信号损失的60％）中都受到温度变化（-21摄氏度至+58摄氏度）和存储的显着影响。在我们的分析中，这引起了适体信号的巨大变化。但是，我们发现在整个EXPOSE-R2任务完成后，仍有> 50％的适体仍在起作用。我们得出的结论是，基于适体的仪器非常适合在行星体上进行原位分析，但是检测步骤需要额外的研究。

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来源
《Astrobiology》 |2019年第8期|1063-1074|共12页
作者
Coussot Gaelle; Le Postollec Aurelie; Incerti Sebastien; Baque Mickael; Faye Clement; Vandenabeele-Trambouze Odile; Cottin Herve; Ravelet Corinne; Peyrin Eric; Fiore Emmanuelle; Vigier Flavie; Caron Jerome; Chaput Didier; Przybyla Bartos; Berger Thomas; Dobrijevic Michel;
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作者单位

Univ Montpellier, ENSCM, CNRS, IBMM, F-34093 Montpellier 5, France;

Univ Bordeaux, B18N, CNRS, LAB, Pessac, France;

Univ Bordeaux, UMR 5797, CENBG, Gradignan, France;

German Aerosp Ctr DLR, Inst Planetary Res Management & Infrastruct, Res Grp Astrobiol Labs, Berlin, Germany;

Colcom, Cap Alpha, Clapiers, France;

UBO, LMEE, IUEM UMR 6197, Plouzane, France;

Univ Paris Est Creteil, UMR 7583, LISA, Creteil, France|Univ Paris Diderot, Inst Pierre Simon Lapl, Creteil, France;

Univ Grenoble Alpes, Dept Pharmacochim Mol, CNRS, UMR 5063, St Martin Dheres, France;

Univ Grenoble Alpes, Dept Pharmacochim Mol, CNRS, UMR 5063, St Martin Dheres, France;

Univ Grenoble Alpes, Dept Pharmacochim Mol, CNRS, UMR 5063, St Martin Dheres, France;

Comprehens Canc Ctr, Inst Bergonie, Dept Radiotherapie, Bordeaux, France;

Ctr Natl Etud Spatiales, DCT ME EM, Toulouse, France;

German Aerosp Ctr, Inst Aerosp Med, Cologne, Germany;

German Aerosp Ctr, Inst Aerosp Med, Cologne, Germany;

Univ Bordeaux, B18N, CNRS, LAB, Pessac, France;

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收录信息美国《科学引文索引》(SCI);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
Astrobiology; Cosmic rays; Biochip; Aptamers;

机译：天体学;宇宙射线;生物芯片;适体;

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专利

1. Photochemistry on the Space Station-Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station [J] . Coussot Gaelle, Le Postollec Aurelie, Incerti Sebastien, Astrobiology . 2019,第8期

机译：空间站 - 适体抵抗空间条件的光化学：粒子照射设施和国际空间站外的真正曝光
2. Photochemistry on the Space Station-Antibody Resistance to Space Conditions after Exposure Outside the International Space Station [J] . Coussot Gaelle, Le Postollec Aurelie, Faye Clement, Astrobiology . 2019,第8期

机译：空间站上的光化学-暴露于国际空间站外的抗体对空间条件的抵抗力
3. Photochemistry on the Space Station-Antibody Resistance to Space Conditions after Exposure Outside the International Space Station [J] . Coussot Gaelle, Le Postollec Aurelie, Faye Clement, Astrobiology . 2019,第8期

机译：国际空间站外曝光后空间站 - 抗体对空间条件的光化学
4. Space hands-on universe telescope and orbiting wide-angle light-ollector telescope to be built on the japanese experiment module exposure facility of the international space station [C] . Yoshiyuki Takahashi. Toshikazu Ebisuzaki, Carlton Pennypacker, American Institute of Physics Conference on international space station utilization . 1999

机译：空间实践宇宙望远镜和轨道广角光集电极望远镜，建于国际空间站的日本实验模块曝光设施
5. A model for predicting radiation exposure for mission planning aboard the International Space Station. [D] . EI-Jaby, Samy. 2012

机译：用于国际空间站上任务计划的辐射暴露预测模型。
6. Microbiomes of the dust particles collected from the International Space Station and Spacecraft Assembly Facilities [O] . Aleksandra Checinska, Alexander J. Probst, Parag Vaishampayan, 2015

机译：从国际空间站和航天器组装设施收集的尘埃颗粒的微生物群
7. Photochemistry on the Space Station—Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station [O] . Gaëlle Coussot, Aurélie Le Postollec, Sébastien Incerti, 2019

机译：空间站 - 适体抵抗空间条件的光化学：粒子照射设施和国际空间站外的真正曝光
8. Status: Crewmember Noise Exposures on the International Space Station. [R] . Limardo-Rodriguez, J. G., Allen, C. S., Danielson, R. W. 2015

机译：现状：国际空间站上的船员噪声暴露。

Photochemistry on the Space Station-Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station

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