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首页> 外文期刊>Astrobiology >Recurrent Isolation of Hydrogen Peroxide-Resistant Spores of Bacillus pumilus from a Spacecraft Assembly Facility
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Recurrent Isolation of Hydrogen Peroxide-Resistant Spores of Bacillus pumilus from a Spacecraft Assembly Facility

机译：从航天器组装设施中反复分离出耐过氧化氢的芽孢杆菌芽孢。

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While the microbial diversity of a spacecraft assembly facility at the Jet Propulsion Laboratory (Pasadena, CA) was being monitored, H_2O_2-resistant bacterial strains were repeatedly isolated from various surface locations. H_2O_2 is a possible sterilant for spacecraft hardware because it is a low-temperature process and compatible with various modern-day spacecraft materials, electronics, and components. Both conventional biochemical testing and molecular analyses identified these strains as Bacillus pumilus. This Bacillus species was found in both unclassified (entrance floors, anteroom, and air-lock) and classified (floors, cabinet tops, and air) locations. Both vegetative cells and spores of several B. pumilus isolates were exposed to 5% liquid H_2O_2 for 60 min. Spores of each strain exhibited higher resistance than their respective vegetative cells to liquid H_2O_2. Results indicate that the H_2O_2 resistance observed in both vegetative cells and spores is strain-specific, as certain B. pumilus strains were two to three times more resistant than a standard Bacillus subtilis dosimetry strain. An example of this trend was observed when the type strain of B. pumilus, ATCC 7061, proved sensitive, whereas several environmental strains exhibited varying degrees of resistance, to H_2O_2. Repeated isolation of H_2O_2-resistant strains of B. pumilus in a clean-room is a concern because their persistence might potentially compromise life-detection missions, which have very strict cleanliness and sterility requirements for spacecraft hardware.
机译：在监测喷气推进实验室（加利福尼亚州帕萨迪纳）的航天器组装设施的微生物多样性的同时，从各个表面位置反复分离出了耐H_2O_2的细菌菌株。 H_2O_2是航天器硬件的一种可能的消毒剂，因为它是低温过程，并且与各种现代航天器材料，电子设备和组件兼容。常规的生化测试和分子分析均将这些菌株鉴定为短小芽孢杆菌。该芽孢杆菌属菌种在未分类（入口层，前室和气密室）和已分类（地板，橱柜顶和空气）位置均被发现。将几种短小芽孢杆菌分离株的营养细胞和孢子都暴露于5％液态H_2O_2中60分钟。每个菌株的孢子表现出比其各自的营养细胞更高的对液体H_2O_2的抗性。结果表明，在营养细胞和孢子中均观察到H_2O_2抗性是菌株特异性的，因为某些短小芽孢杆菌菌株的抗性比标准枯草芽孢杆菌剂量法菌株高2至3倍。当证明短小芽孢杆菌的类型菌株ATCC 7061敏感时，观察到这种趋势的一个例子，而几种环境菌株对H_2O_2表现出不同程度的抗性。在洁净室中反复分离耐H_2O_2的短小芽孢杆菌菌株是一个问题，因为它们的持久性可能会损害生命探测任务，这对航天器硬件有非常严格的清洁度和无菌要求。

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