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Transcription profiles of Streptococcus pneumoniae grown under different conditions of normal gravitation

机译:在正常引力的不同条件下生长的肺炎链球菌的转录特征

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High-aspect rotating vessels (HARVs) are used to study the effects low-shear modeled microgravity (LSMMG) on bacterial gene expression. LSMMG is generated by orienting HARVs with the axis of rotation perpendicular to the gravity vector while gravitational controls are oriented with the axis of rotation parallel to the gravity vector. Microarray analysis was performed on Streptococcus pneumoniae TIGR4 grown in HARVs under three conditions (LSMMG, 1 x g, and static) to determine if global transcriptional activity is altered between different gravitational controls and LSMMG. Results revealed 101 differentially expressed genes under static conditions compared to 1 x g, 46 genes between 1 x g and LSMMG, and nine genes between static and LSMMG. Hierarchical cluster analysis revealed 15 genes exhibiting similar expression patterns under static conditions compared to 1 x g. These results indicate that rotation, in addition to low-shear forces, might contribute to bacterial adaptation to the LSMMG.
机译:高纵横旋转容器(HARVs)用于研究低剪切模型微重力(LSMMG)对细菌基因表达的影响。 LSMMG是通过使HARV的旋转轴垂直于重力矢量而生成的,而重力控制的旋转轴则平行于重力矢量。在三种条件下(LSMMG,1 x g和静态),对在HARV中生长的肺炎链球菌TIGR4进行了微阵列分析,以确定在不同的引力对照和LSMMG之间全局转录活性是否发生了改变。结果显示在静态条件下101个差异表达基因与1 x g,1 x g和LSMMG之间的46个基因以及在静态和LSMMG之间的9个基因相比。层次聚类分析显示,在静态条件下与1 x g相比,有15个基因表现出相似的表达模式。这些结果表明,除了低剪切力外,旋转还可能有助于细菌适应LSMMG。

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