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首页> 外文期刊>Biochemical Genetics >Genotyping an ALAD Polymorphism with Real-Time PCR in Two Populations from the Iberian Peninsula
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Genotyping an ALAD Polymorphism with Real-Time PCR in Two Populations from the Iberian Peninsula

机译：利用实时PCR对伊比利亚半岛的两个种群进行ALAD多态性基因分型

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Lead-induced toxicity varies among individuals partly because of genetic differences in their susceptibility to the metal’s effects. One genetic polymorphism associated with lead toxicity is a G-to-C transversion at position 177 in the coding region of delta-aminolevulinic acid dehydratase (ALAD), originating from two codominant alleles (ALAD1 and ALAD2). We examined the distribution of this single nucleotide polymorphism in two populations from the Iberian Peninsula. Genomic DNA was extracted from whole blood, and a real-time PCR assay was designed to determine ALAD polymorphic distribution. The distribution of MspI polymorphism was similar in the two populations studied, and allelic frequencies were comparable to those obtained in other studies of Caucasians. Further studies are needed to assess fully the functional significance of this polymorphism and its influence on the toxicokinetics of lead.
机译：铅引起的毒性因个体而异，部分原因是他们对金属的敏感性的遗传差异。与铅毒性相关的一种遗传多态性是δ-氨基乙酰丙酸脱水酶（ALAD）编码区中第177位的G-C转换，其起源于两个显性等位基因（ALAD1和ALAD2）。我们检查了来自伊比利亚半岛的两个种群中这种单核苷酸多态性的分布。从全血中提取基因组DNA，并设计了实时PCR测定法来确定ALAD多态性分布。在两个研究人群中，MspI多态性的分布相似，等位基因频率与其他白种人研究中的等位基因频率相当。需要进一步研究，以充分评估这种多态性的功能意义及其对铅毒代动力学的影响。

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