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Genetic Relationships Within and Between Capsicum Species

机译：辣椒种内和种间的遗传关系

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摘要

Genetic relationships were estimated among 24 accessions belonging to 11 species of Capsicum, using 2,760 RAPD markers based on touch-down polymerase chain reactions (Td-RAPD-PCR). These markers were implemented in analyses of principal coordinates, unweighted pair group mean average, and 2,000 bootstrap replications. The accessions were divided into four groups, corresponding to previously described Capsicum complexes: C. annuum complex (CA), C. baccatum complex (CB), C. pubescens complex (CP), and C. chacoense accessions (CA/B). Their overall mean genetic similarity index was 0.487 ± 0.082, ranging from 0.88 to 0.32, based on Jaccard’s coefficient. The highest genetic variation was observed among the accessions in CP; the accessions in CB had a low level of variation as judged from the standard deviations of the genetic similarity indices. Based on the Td-RAPD-PCR markers, the 24 accessions were divided into four major groups, three of which corresponded to the three distinct Capsicum complexes. Accessions of C. chacoense were found to be equally related to complexes CA, CB, and CP. Keywords RAPD-PCR - DNA markers - Species relationships - Capsicum complex - Touch-down PCR
机译：使用基于触地聚合酶链反应（Td-RAPD-PCR）的2,760 RAPD标记，估计了属于11个辣椒的24个种质之间的遗传关系。这些标记是通过分析主坐标，未加权对组的平均数和2,000个引导程序复制来实现的。这些种质被分为四类，分别对应于先前描述的辣椒复合物：C.anumum复合物（C A ），B.bacatum复合物（C B ），C。pubescens。复杂（C P ）和C. chacoense种（C A / B ）。根据雅卡德系数，他们的整体平均遗传相似性指数为0.487±0.082，范围从0.88到0.32。 C P 的种质间遗传变异最高。根据遗传相似性指标的标准差判断，C B 中的种质变异程度较低。根据Td-RAPD-PCR标记，将24个种质分为四个主要组，其中三个对应于三个不同的辣椒素复合物。发现Chacoense的种质与复合物C A ，C B 和C P 均等相关。关键词RAPD-PCR-DNA标记-种间关系-辣椒复合物-落地PCR

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