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Identification and Characteristics of Cattle MicroRNAs by Homology Searching and Small RNA Cloning

机译：通过同源搜索和小RNA克隆鉴定和鉴定牛MicroRNAs

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摘要

MicroRNAs (miRNA) are a class of noncoding RNA molecules that regulate gene expression by an RNA-interfering pathway through cleavage or inhibition of the translation of target mRNA. The 254 cattle miRNA candidates found by homology searching frequently clustered at certain chromosomes, and some are possibly expressed from more than one genomic locus. They were partially verified by cloning from a small cattle RNA library, where 31 distinct miRNAs were identified: 18 previously registered in the database of miRBase, 11 novel and homologous to known mammalian miRNAs, and 2 potentially novel without homology to any known miRNAs. Partial miRNA expression was detected by RT-PCR in cattle tissues, such as brain, liver, lung, and heart; some were expressed in all tissues and others in a specific tissue. Sequence alignments revealed that many had end variants, most of which differed in the 3′ end; a small number differed in the 5′ end. This indicates that the same miRNA gene can be individually modified in the process of miRNA biogenesis and could have a different role in regulating target gene expression. Keywords MicroRNA - Cattle - Homology searching - Small RNA cloning
机译：微小RNA（miRNA）是一类非编码RNA分子，通过切割或抑制靶mRNA的翻译而通过RNA干扰途径调节基因表达。通过同源性搜索发现的254个牛miRNA候选物通常聚集在某些染色体上，有些可能是从多个基因组位点表达的。它们是通过从一个小的牛RNA库中克隆而得到部分验证的，其中鉴定了31个不同的miRNA：18个先前在miRBase数据库中注册，11个与已知哺乳动物miRNA同源且与之同源，以及2个与任何已知miRNA没有同源性的潜在新颖。通过RT-PCR在牛组织（如脑，肝，肺和心脏）中检测到部分miRNA的表达；一些在所有组织中表达，而另一些在特定组织中表达。序列比对显示许多具有末端变体，其中大多数在3'末端有所不同。少数在5'端有所不同。这表明相同的miRNA基因可以在miRNA生物发生过程中被单独修饰，并且在调节靶基因表达中可能具有不同的作用。关键词MicroRNA-牛-同源性搜索-小RNA克隆

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