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Molecular Cloning, Mapping, and Expression Analysis of the EIF4A2 Gene in Pig

机译：猪EIF4A2基因的分子克隆，定位及表达分析

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摘要

A full-length cDNA clone encoding the eukaryotic translation initiation factor 4A, isoform 2 (EIF4A2), was cloned from the fetal skeletal cDNA library from the pig (Sus scrofa). EIF4A2 is a highly conserved gene for one of the protein-synthesis initiation factors involved in the binding of mRNA to the ribosome. Based on this cDNA sequence, the deduced protein of 407 amino acids contains the characteristic motifs shared by the DEAD-box supergene family. The genomic nucleotide sequence of this gene was determined and a single nucleotide polymorphism located in the 5′ untranslated region was genotyped. The porcine EIF4A2 was expressed in all tissues examined but in variable amounts. The EIF4A2 expression level in muscle was upregulated through embryonic and neonatal development until adult, suggesting that porcine EIF4A2 was possibly involved in translation regulation of other muscle-related genes in muscle formation and development. In addition, we mapped porcine EIF4A2 to q4.1 of SSC13, in agreement with comparative mapping data.
机译：从猪（Sus scrofa）的胎儿骨骼cDNA文库中克隆了一个编码真核翻译起始因子4A同工型2（EIF4A2）的全长cDNA克隆。 EIF4A2是涉及mRNA与核糖体结合的一种蛋白质合成起始因子的高度保守基因。根据该cDNA序列，推导的407个氨基酸的蛋白质包含DEAD-box超基因家族共有的特征性基序。确定该基因的基因组核苷酸序列，并对位于5'非翻译区的单核苷酸多态性进行基因分型。猪EIF4A2在所有检查过的组织中均表达，但数量可变。直到成年，通过胚胎和新生儿发育，肌肉中EIF4A2的表达水平均被上调，这表明猪EIF4A2可能参与了肌肉形成和发育过程中其他与肌肉相关的基因的翻译调控。此外，我们将猪EIF4A2映射到SSC13的q4.1，与比较映射数据一致。

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  • 来源
    《Biochemical Genetics》 |2007年第2期|51-62|共12页
  • 作者

    Heng Wang; Huanling Wang; Zhengmao Zhu; Shulin Yang; Kui Li;

  • 作者单位

    Key Laboratory of Animal Genetics Breeding and Reproduction of the Ministry of Education of China Huazhong Agricultural University Wuhan 430070 P.R. China;

    Key Laboratory of Animal Genetics Breeding and Reproduction of the Ministry of Education of China Huazhong Agricultural University Wuhan 430070 P.R. China;

    Department of Gene and Cell Engineering Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100094 P.R. China;

    Department of Gene and Cell Engineering Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100094 P.R. China;

    Department of Gene and Cell Engineering Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100094 P.R. China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    chromosome location; EIF4A2; expression pattern; polymorphism; porcine;

    机译：染色体定位EIF4A2表达模式多态性猪;

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