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首页> 外文期刊>Biochemical Genetics >Genetic Differentiation of the Giant Honey Bee (Apis dorsata) in Thailand Analyzed by Mitochondrial Genes and Microsatellites
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Genetic Differentiation of the Giant Honey Bee (Apis dorsata) in Thailand Analyzed by Mitochondrial Genes and Microsatellites

机译：线粒体基因和微卫星技术分析泰国巨型蜜蜂的遗传分化

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摘要

Genetic diversity and population differentiation of the giant honey bee (Apis dorsata) in Thailand were examined. Six PCR-RFLP mitotypes were generated from digestion of the COI-COII, Cytb-tRNAser, ATPase6-8, and lrRNA genes with Dra I and Hin fI. Low genetic diversity (h=0.074, π=0.032%) and a lack of genetic population differentiation between A. dorsata originating from geographically different regions were observed from mtDNA polymorphisms (P > 0.05). In contrast, microsatellite (A14, A24, and A88) polymorphisms revealed a relatively high level of genetic diversity in A. dorsata (H o=0.68–0.74, average number of alleles per locus=6.0–9.0). Both A24 and A88 indicated significant population differentiation between bees from the north-to-central region (north, northeast, and central regions), peninsular Thailand, and Samui Island.
机译：研究了泰国巨型蜜蜂（Apis dorsata）的遗传多样性和种群分化。通过用Dra I和Hin fI消化COI-COII，Cytb-tRNAser ，ATPase6-8和lrRNA基因产生了6种PCR-RFLP基因型。从mtDNA多态性观察到遗传多样性低（h = 0.074，π= 0.032％）和起源于地理上不同区域的背果之间缺乏遗传种群分化（P> 0.05）。相比之下，微卫星（A14，A24和A88）多态性揭示了背果曲霉的遗传多样性水平较高（H osub = 0.68-0.74，每个基因座的平均等位基因数= 6.0-9.0）。 A24和A88均表明，北至中部地区（北部，东北部和中部地区），泰国半岛和苏梅岛的蜜蜂之间存在明显的种群分化。

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  • 来源
    《Biochemical Genetics》 |2007年第4期|345-361|共17页
  • 作者

    S. Insuan; S. Deowanish; S. Klinbunga; S. Sittipraneed; H. A. Sylvester; S. Wongsiri;

  • 作者单位

    Department of Biochemistry Faculty of Science Chulalongkorn University Bangkok 10330 Thailand;

    Department of Biology Faculty of Science Chulalongkorn University Bangkok 10330 Thailand;

    National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency 113 Paholyothin Road Klong 1 Klong Luang Pathumthani 12120 Thailand;

    Department of Biochemistry Faculty of Science Chulalongkorn University Bangkok 10330 Thailand;

    USDA ARS Honey-Bee Breeding Genetics and Physiology Laboratory 1157 Ben Hur Road Baton Rouge LA 70820 USA;

    Department of Biology Faculty of Science Chulalongkorn University Bangkok 10330 Thailand;

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  • 正文语种 eng
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  • 关键词

    Apis dorsata; honey bee; genetic diversity; mtDNA; microsatellites;

    机译：蜜蜂;蜜蜂;遗传多样性;mtDNA;微卫星;

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