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首页> 外文期刊>Biochemical Genetics >Molecular cloning, mapping, and tissue expression of the porcine cluster of differentiation 14 (CD14) gene
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Molecular cloning, mapping, and tissue expression of the porcine cluster of differentiation 14 (CD14) gene

机译：猪分化14（CD14）基因簇的分子克隆，作图和组织表达

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摘要

CD14 plays an important role in initiating the innate response to lipopolysaccharide from Gram-negative bacteria. The gene and corresponding cDNA of porcine CD14 were sequenced and characterized. The porcine CD14 gene consists of two exons and a short intron (80 bp) located immediately after the ATG translation start codon. This structure is very similar to the CD14 gene of human, rat, mouse, rabbit, horse, and cow. The sequence of the porcine CD14 protein is 59–76% identical to that of rat, mouse, rabbit, human, horse, and cow CD14 protein. A highly conserved structure of the CD14 protein with respect to the leucine-rich repeats domain and the N-glycosylation sites was observed between species. Porcine CD14 was assigned to porcine chromosome 2q21 by a radiation hybrid panel. Using RT-PCR analysis, porcine CD14 transcripts were detected in liver, spleen, thymus, white matter, and skeletal muscle.
机译：CD14在引发对革兰氏阴性细菌对脂多糖的先天应答中起重要作用。对猪CD14的基因和相应的cDNA进行测序和鉴定。猪CD14基因由两个外显子和一个短内含子（80 bp）组成，位于ATG翻译起始密码子之后。这种结构与人，大鼠，小鼠，兔，马和牛的CD14基因非常相似。猪CD14蛋白的序列与大鼠，小鼠，兔，人，马和牛CD14蛋白的序列具有59–76％的同一性。在物种之间观察到相对于富含亮氨酸的重复结构域和N-糖基化位点，CD14蛋白的高度保守的结构。放射杂交小组将猪CD14分配给猪染色体2q21。使用RT-PCR分析，在肝脏，脾脏，胸腺，白质和骨骼肌中检测到猪CD14转录本。

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  • 来源
    《Biochemical Genetics》 |2007年第6期|459-468|共10页
  • 作者

    Xiao-Tian Qiu; Yu-Hua Li; HeJun Li; Ying Yu; Qin Zhang;

  • 作者单位

    Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture College of Animal Science and Technology China Agricultural University Beijing P.R. China;

    Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture College of Animal Science and Technology China Agricultural University Beijing P.R. China;

    Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture College of Animal Science and Technology China Agricultural University Beijing P.R. China;

    Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture College of Animal Science and Technology China Agricultural University Beijing P.R. China;

    Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture College of Animal Science and Technology China Agricultural University Beijing P.R. China;

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  • 正文语种 eng
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  • 关键词

    CD14; Innate immunity; Leucine-rich repeats; Radiation hybrid panel; Gene expression;

    机译：CD14;先天免疫;富亮氨酸重复序列;辐射杂交组;基因表达;

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