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首页> 外文期刊>Biochemical Genetics >Genetic Structure of Different Populations of Walking Catfish (Clarias batrachus L.) in Bangladesh
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Genetic Structure of Different Populations of Walking Catfish (Clarias batrachus L.) in Bangladesh

机译：孟加拉国Walking鱼（Clarias batrachus L.）不同种群的遗传结构

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Information on genetic variation is essential for conservation and stock improvement programs. Seven dinucleotide microsatellite loci were analyzed to reveal genetic variability in three wild populations (Kella beel, Hakaluki haor, and Shobornokhali beel) and one hatchery population of the freshwater walking catfish, Clarias batrachus, in Bangladesh. Upon PCR amplification, the alleles were separated on polyacrylamide gel using a sequencing gel electrophoresis system and visualized by the silver-staining method. The loci were polymorphic (P 95) in all the populations. Differences were observed in number and frequency of alleles as well as heterozygosity in the studied populations. Current gene diversity (H e ) was higher than expected under mutation-drift equilibrium, significantly in the Hakaluki haor and Shobornokhali beel populations, indicating a recent genetic bottleneck. Population differentiation (F ST) values were significant (P < 0.05) in all the population pairs. A relatively high level of gene flow and a low level of F ST values were found between wild population pairs compared to hatchery-wild pairs. The unweighted pair group method with averages dendrogram based on genetic distance resulted in two major clusters: the hatchery population was alone in one cluster whereas the three wild populations made another cluster. The results reflect some degree of genetic variability in C. batrachus populations indicating potentialities for improving this species through a selective breeding program. The results revealed a recent bottleneck in some wild populations of C. batrachus. Protection of habitat may help increase the population size and lower the risk of vulnerability of the species in the future.
机译：有关遗传变异的信息对于保护和种群改良计划至关重要。分析了七个二核苷酸微卫星基因座，以揭示孟加拉国的三个野生种群（凯拉比尔，Hakaluki haor和Shobornokhali beel）和一个孵化场种群的淡水walking鱼Clarias batrachus的遗传变异性。 PCR扩增后，使用测序凝胶电泳系统在聚丙烯酰胺凝胶上分离等位基因，并通过银染色法观察。在所有人群中，基因座均为多态性（P 95 ）。在研究人群中观察到等位基因的数量和频率以及杂合性的差异。在突变漂移平衡下，当前的基因多样性（H ）高于预期，在哈卡卢基（Hakaluki haor）和肖邦克哈利（Shobornokhali）的beel种群中显着，表明了最近的遗传瓶颈。在所有种群对中，种群分化（F ST ）值均显着（P <0.05）。与孵化场-野生对相比，野生种群对之间的基因流动水平相对较高，F ST 值较低。基于遗传距离的平均树形图的非加权对群方法产生了两个主要的集群：孵化场种群一个集群，而三个野生种群又一个集群。结果反映了非洲梭菌种群的某种程度的遗传变异性，表明通过选择性育种程序改良该物种的潜力。结果揭示了一些野生巴氏梭菌种群的瓶颈。保护生境可能有助于增加种群数量，并降低该物种未来的脆弱性风险。

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