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首页> 外文期刊>Biochemical Genetics >Species-Specific Allozyme Markers for Appalachian Wood-Feeding Cockroaches (Dictyoptera: Cryptocercidae)
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Species-Specific Allozyme Markers for Appalachian Wood-Feeding Cockroaches (Dictyoptera: Cryptocercidae)

机译：用于阿巴拉契亚饲喂蟑螂的物种特定的同工酶标记（双翅目：隐尾科）

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摘要

Members of the genus Cryptocercus are wood-feeding cockroaches that live in the temperate forests. Nine species are recognized in the genus worldwide: two in eastern Eurasia, two in China, and five in the United States. Within the United States, one species occurs in the Pacific Northwest and four occur in the Appalachian Mountains. Previous studies have revealed the presence of potential zones of overlap in distribution among the Appalachian species, raising the possibility of hybridization among them. Differences in mitochondrial DNA have previously been identified for the Appalachian species. However, to identify hybrid individuals one or more species-specific, codominant nuclear markers are required. Therefore, our objective was to undertake allozyme analysis of enzymatic loci to identify fixed, species-specific alleles for the four Appalachian species. We assayed a mean of 42 individuals each from 16 sites for allozyme variation for the four species. At 6 of the 33 loci examined, fixed alternate alleles were identified; a combination of 2 loci enabled the identification of all four species. To identify hybrids in the field, we examined 42 individuals each from 13 sites in which two or more of the above species occur in close proximity for presence of heterozygous individuals at one or more of the six fixed loci. No heterozygous individuals were found suggesting the lack of hybridization among the Appalachian species.
机译：隐尾藻属的成员是生活在温带森林中的食木蟑螂。全世界共有9种：欧亚大陆东部有2种，中国有2种，美国有5种。在美国境内，一种物种出现在西北太平洋，而四种则出现在阿巴拉契亚山脉。先前的研究表明，阿巴拉契亚物种之间存在分布重叠的潜在区域，从而增加了它们之间杂交的可能性。先前已经确定了阿巴拉契亚物种的线粒体DNA的差异。然而，为了鉴定杂种个体，需要一种或多种物种特异性的共性核标记。因此，我们的目标是对酶基因座进行同工酶分析，以鉴定四个阿巴拉契亚物种固定的，物种特异性的等位基因。我们分析了来自16个位点的42个个体的平均值，分析了这4个物种的同工酶变异。在检查的33个基因座中有6个被鉴定出固定的等位基因。 2个基因座的组合可以识别所有四个物种。为了鉴定该领域的杂种，我们检查了来自13个位点的42个个体，其中六个或多个固定位点中的一个或多个位点存在两个或多个以上物种，这些物种非常接近。未发现杂合个体，表明阿巴拉契亚物种之间缺乏杂交。

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