Strand Annealing and Terminal Transferase Activities of a B-family DNA Polymerase

Zhongfeng Zuo Hsiang-Kai Lin and Michael A. Trakselis

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Strand Annealing and Terminal Transferase Activities of a B-family DNA Polymerase

机译：B族DNA聚合酶的链退火和末端转移酶活性

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DNA replication polymerases have the inherent ability to faithfully and rapidlyncopy a DNA template according to precise Watsonu0002Crick base pairing. The primary B-familynDNA replication polymerase (Dpo1) in the hyperthermophilic archaeon, Sulfolobus solfataricus,nis shown here to possess a remarkable DNA stabilizing ability for maintaining weak base pairingninteractions to facilitate primer extension. This thermal stabilization by Dpo1 allowed forntemplate-directed synthesis at temperatures more than 30 u0001C above the melting temperature ofnnaked DNA. Surprisingly, Dpo1 also displays a competing terminal deoxynucleotide transferasen(TdT) activity unlike any other B-family DNA polymerase. Dpo1 is shown to elongate singlestrandednDNA in template-dependent and template-independent manners. Experiments withndifferent homopolymeric templates indicate that initial deoxyribonucleotide incorporation isncomplementary to the template. Rate-limiting steps that include looping back and annealing to the template allow for a uniquentemplate-dependent terminal transferase activity. Themultiple activities of this unique B-family DNA polymerase make this enzymenan essential component for DNA replication and DNA repair for the maintenance of the archaeal genome at high temperatures.

机译：DNA复制聚合酶具有根据精确的Watsonu0002Crick碱基配对忠实，快速复制DNA模板的固有能力。嗜热古细菌Sulfolobus solfataricus中的主要B-家族基因复制聚合酶（Dpo1）在这里显示具有显着的DNA稳定能力，可以维持弱碱基配对相互作用，以促进引物延伸。 Dpo1的这种热稳定作用允许在高于掺入DNA的熔解温度30 u0001C以上的温度进行模板指导的合成。出人意料的是，与任何其他B族DNA聚合酶不同，Dpo1还显示出竞争性的末端脱氧核苷酸转移酶（TdT）活性。 Dpo1被证明以依赖模板和不依赖模板的方式延长单链DNA。使用不同均聚物模板的实验表明，最初的脱氧核糖核苷酸掺入与模板互补。包括环回和退火至模板的限速步骤可实现独特的依赖于模板的末端转移酶活性。这种独特的B族DNA聚合酶的多种活性使该酶成为DNA复制和DNA修复中不可或缺的组成部分，以在高温下维持古细菌基因组。

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《Biochemistry》 |2011年第23期|p.5379-5390|共12页
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Zhongfeng Zuo Hsiang-Kai Lin and Michael A. Trakselis;
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Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States;

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1. Strand Annealing and Terminal Transferase Activities of a B-family DNA Polymerase [J] . Zuo ZF, Lin HK, Trakselis MA Biochemistry . 2011,第23期

机译：B族DNA聚合酶的链退火和末端转移酶活性
2. Detection of single- and double-strand DNA breaks after traumatic brain injury in rats: comparison of in situ labeling techniques using DNA polymerase I, the Klenow fragment of DNA polymerase I, and terminal deoxynucleotidyl transferase. [J] . Clark RSB, nes.upmc.edu, Chen M, Journal of neurotrauma . 2001,第7期

机译：大鼠脑外伤后单链和双链DNA断裂的检测：使用DNA聚合酶I，DNA聚合酶I的Klenow片段和末端脱氧核苷酸转移酶的原位标记技术的比较。
3. Mutagenesis of human DNA polymerase lambda: essential roles of Tyr505 and Phe506 for both DNA polymerase and terminal transferase activities [J] . Shevelev I, Blanca G, Villani G, Nucleic Acids Research . 2003,第23期

机译：人类DNA聚合酶λ的诱变：Tyr505和Phe506对DNA聚合酶和末端转移酶活性的重要作用
4. DNA Length and Cationic Cofactor Dependent Strand Transfer Activity of HIV-1 Integrase [C] . Hong-qiu He, Cun-xin Wang, Wei-zu Chen Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009 . 2009

机译：HIV-1整合酶的DNA长度和阳离子辅因子依赖性链转移活性。
5. The DNA polymerase III holoenzyme: An asymmetric dimeric replicative complex containing distinguishable leading and lagging strand polymerases. [D] . Glover, Bradley P. 2001

机译：DNA聚合酶III全酶：一种不对称的二聚体复制复合体，其中包含可区分的前导链和滞后链聚合酶。
6. Mutagenesis of human DNA polymerase λ: essential roles of Tyr505 and Phe506 for both DNA polymerase and terminal transferase activities [O] . Igor Shevelev, Giuseppina Blanca, Giuseppe Villani, 2003

机译：人类DNA聚合酶λ的诱变：Tyr505和Phe506对DNA聚合酶和末端转移酶活性的重要作用
7. DNA elongation by the human DNA polymerase lambda polymerase and terminal transferase activities are differentially coordinated by proliferating cell nuclear antigen and replication protein A. [O] . Maga, G, Ramadan, K, Locatelli, GA, 2005

机译：人类DNA聚合酶λ聚合酶的DNA延伸和末端转移酶的活性通过增殖细胞核抗原和复制蛋白A来不同地协调。

Strand Annealing and Terminal Transferase Activities of a B-family DNA Polymerase

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