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首页> 外文期刊>Biochemistry >NMR Characterization of the Interaction of the Salmonella Type III Secretion System Protein SipD and Bile Salts,
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NMR Characterization of the Interaction of the Salmonella Type III Secretion System Protein SipD and Bile Salts,

机译：沙门氏菌III型分泌系统蛋白SipD和胆汁盐相互作用的NMR表征，

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摘要

Salmonella and Shigella bacteria require the type III secretion system (T3SS) to inject virulencenproteins into their hosts and initiate infections. The tip proteins SipDand IpaDare critical components of thenSalmonella and Shigella T3SS, respectively. Recently, SipD and IpaD have been shown to interact with bilensalts, which are enriched in the intestines, and are hypothesized to act as environmental sensors for thesenenteric pathogens. Bile salts activate the Shigella T3SS but repress the Salmonella T3SS, and themechanismofnthis differing response to bile salts is poorly understood. Further, how SipD binds to bile salts is currentlynunknown. Computer modeling predicted that IpaD binds the bile salt deoxycholate in a cleft formed by thenN-terminal domain and the long central coiled coil of IpaD.Here, we usedNMRmethods to determine whichnSipD residues are affected by the interaction with the bile salts deoxycholate, chenodeoxycholate, andntaurodeoxcholate. The bile salts perturbed nearly the same set of SipDresidues; however, the largest chemicalnshift perturbations occurred away from what was predicted for the bile salt binding site in IpaD. Our NMRnresults indicate that that bile salt interaction of SipD will be different from what was predicted for IpaD,nsuggesting a possible mechanism for the differing response of Salmonella and Shigella to bile salts.
机译：沙门氏菌和志贺氏菌需要III型分泌系统（T3SS），才能将毒力蛋白注入其宿主并引发感染。提示蛋白SipD和IpaD分别是当时的沙门氏菌和志贺氏菌T3SS的关键成分。最近，SipD和IpaD已显示出与在肠道中富集的胆石盐相互作用，并被认为可作为敏感病原体的环境传感器。胆汁盐激活了志贺氏菌T3SS，但抑制了沙门氏菌T3SS，这种对胆盐的不同反应的机制尚不清楚。此外，目前尚不知道SipD如何结合胆汁盐。计算机模型预测，IpaD会在I末端的N末端结构域和长的中心螺旋形线圈形成的裂隙中结合胆汁盐脱氧胆酸盐。 。胆汁盐会扰动几乎相同的SipDresidues。然而，最大的化学位移扰动发生在远离IpaD中胆盐结合位点的预测值上。我们的NMR结果表明，SipD的胆盐相互作用将不同于对IpaD的预测，这暗示了沙门氏菌和志贺氏菌对胆盐的不同反应的可能机制。

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