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首页> 外文期刊>Biochemistry >Elucidating the Catalytic Mechanism of Sulfite Oxidizing Enzymes Using Structural, Spectroscopic, and Kinetic Analyses
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Elucidating the Catalytic Mechanism of Sulfite Oxidizing Enzymes Using Structural, Spectroscopic, and Kinetic Analyses

机译：使用结构，光谱和动力学分析阐明亚硫酸盐氧化酶的催化机理

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摘要

Sulfite oxidizing enzymes (SOEs) are molybdenum cofactor-dependent enzymes that are found innplants, animals, and bacteria. Sulfite oxidase (SO) is found in animals and plants, while sulfite dehydrogenasen(SDH) is found in bacteria. In animals, SO catalyzes the oxidation of toxic sulfite to sulfate as the final step innthe catabolism of the sulfur-containing amino acids, methionine and cysteine. In humans, sulfite oxidasendeficiency is an inherited recessive disorder that produces severe neonatal neurological problems that lead tonearly death. Plant SO (PSO) also plays an important role in sulfite detoxification and in addition serves as annintermediate enzyme in the assimilatory reduction of sulfate. In vertebrates, the proposed catalyticmechanismnof SO involves two intramolecular one-electron transfer (IET) steps from the molybdenum cofactor to theniron of the integral b-type heme. A similar mechanism is proposed for SDH, involving its molybdenumncofactor and c-type heme.However, PSO, which lacks an integral heme cofactor, uses molecular oxygen as itsnelectron acceptor. Here we review recent results for SOEs from kinetic measurements, computational studies,nelectron paramagnetic resonance (EPR) spectroscopy, electrochemical measurements, and site-directednmutagenesis on active site residues of SOEs and of the flexible polypepetide tether that connects the hemenand molybdenum domains of human SO. Rapid kinetic studies of PSO are also discussed.
机译：亚硫酸盐氧化酶（SOE）是钼辅因子依赖性酶，存在于植物，动物和细菌中。在动物和植物中发现了亚硫酸盐氧化酶（SO），而在细菌中发现了亚硫酸盐脱氢酶（SDH）。在动物中，SO催化有毒亚硫酸盐氧化为硫酸盐，这是含硫氨基酸，蛋氨酸和半胱氨酸分解代谢的最后一步。在人类中，亚硫酸盐氧化酶缺乏症是一种遗传性隐性疾病，会引起严重的新生儿神经系统疾病，导致音质死亡。植物SO（PSO）在亚硫酸盐的解毒中也起着重要的作用，此外在硫酸盐的同化还原中也起着中间酶的作用。在脊椎动物中，拟议的SO催化机理涉及从钼辅因子到整体b型血红素铁的两个分子内单电子转移（IET）步骤。对于SDH，有人提出了类似的机制，涉及其钼辅因子和c型血红素，但缺乏必需的血红素辅因子的PSO使用分子氧作为其电子受体。在这里，我们将从动力学测量，计算研究，电子顺磁共振（EPR）光谱，电化学测量以及SOE活性位点残基和连接人类SO的钼和钼结构域的柔性多肽系链的位点定向诱变的角度回顾SOE的最新结果。还讨论了PSO的快速动力学研究。

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